RGD Reference Report - Osteopontin in kainic acid-induced microglial reactions in the rat brain. - Rat Genome Database

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Osteopontin in kainic acid-induced microglial reactions in the rat brain.

Authors: Kim, SY  Choi, YS  Choi, JS  Cha, JH  Kim, ON  Lee, SB  Chung, JW  Chun, MH  Lee, MY 
Citation: Kim SY, etal., Mol Cells. 2002 Jun 30;13(3):429-35.
RGD ID: 1581327
Pubmed: PMID:12132583   (View Abstract at PubMed)

The present study was performed to investigate the spatial and temporal expression of osteopontin (OPN) mRNA in the rat brain after kainic acid-induced seizures, and to determine whether this phenomenon is associated spatiotemporally with the microglial reaction. The expression of OPN mRNA was detected using an in situ hybridization technique and Northern blot analysis. Following intraperitoneal injection of kainic acid (10 mg/kg), OPN mRNA was expressed in selective vulnerable areas, including the hippocampus, thalamus, hypothalamus, amygdala, and entorhinal cortex. Comparison of the morphology and localization with the established microglial marker OX-42 in the adjacent sections positively identified the OPN-expressed cells as microglia. Furthermore, double labeling experiments revealed that OPN mRNA expression was confined to ameboid-like cells among microglia stained with GSI-B4, an another microglial marker. These findings from a rat model of seizure support the notion that OPN can be synthesized in a subpopulation of reactive microglial cells. It can therefore be assumed that in the response of the brain to excitotoxic injury, synthesis of OPN occurs generally in a subset of activated microglia.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
visual epilepsy  ISOSpp1 (Rattus norvegicus)1581327; 1581327 RGD 
visual epilepsy  IDA 1581327 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Spp1  (secreted phosphoprotein 1)

Genes (Mus musculus)
Spp1  (secreted phosphoprotein 1)

Genes (Homo sapiens)
SPP1  (secreted phosphoprotein 1)


Additional Information