RGD Reference Report - Pituitary synaptic protein SNAP-25 sensitive to GnRH is necessary for LH release. - Rat Genome Database

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Pituitary synaptic protein SNAP-25 sensitive to GnRH is necessary for LH release.

Authors: Quintanar, JL  Salinas, E  Chavez-Morales, RM  Quintanar-Stephano, A 
Citation: Quintanar JL, etal., Endocr Regul. 2004 Mar;38(1):1-6.
RGD ID: 1581080
Pubmed: PMID:15147232   (View Abstract at PubMed)

OBJECTIVE: The protein SNAP-25 is located in the plasma membrane and is known to participate in hormone exocytosis process. In the present work we studied the role of SNAP-25 on LH secretion in permeabilized adenohypophyseal cultured cells. The question of whether GnRH regulates SNAP-25 expression in adenohypophyseal cultured cells and in the adenohypophyses in vivo was also investigated. METHODS: In digitonin-permeabilized cells incubated with anti-SNAP-25, stimulated LH secretion with Ca2+ was analysed. The presence and expression of SNAP-25 in adenohypophyseal cultured cells incubated with GnRH and in adenohypophyses of orchidectomized rats with GnRH administration was studied by immunochemistry and immunoblotting. RESULTS: Immunochemical study revealed that SNAP-25 was present in cultured adenohypophyseal cells and in adenohypophysis of orchidectomized rats both with GnRH treatment. We found that LH secretion can be blocked by antibodies raised against SNAP-25 in permeabilized cells. Likewise, GnRH administration induced a significant decrease of SNAP-25 expression in cultured adenohypophyseal cells and in adenohypophysis of orchidectomized rats. CONCLUSION: Our study showed that SNAP-25 is present in adenohypophyses in vitro as well as in vivo and that is involved in LH release and that GnRH can modify its expression.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of hormone secretion  IMP 1581080 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Snap25  (synaptosome associated protein 25)


Additional Information