RGD Reference Report - Calcineurin and matrix protein expression in cardiac hypertrophy : Evidence for calcineurin B to control excessive hypertrophic signaling. - Rat Genome Database

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Calcineurin and matrix protein expression in cardiac hypertrophy : Evidence for calcineurin B to control excessive hypertrophic signaling.

Authors: Grammer, JB  Bleiziffer, S  Monticelli, F  Lange, R  Bauernschmitt, R 
Citation: Grammer JB, etal., Basic Res Cardiol. 2006 Jul;101(4):292-300. Epub 2006 May 10.
RGD ID: 1580706
Pubmed: PMID:16688406   (View Abstract at PubMed)
DOI: DOI:10.1007/s00395-006-0598-z   (Journal Full-text)

In the compensatory state of human left ventricular hypertrophy (LVH), the remodeling processes in the extracellular matrix and the role of calcineurin (Cn) are not completely understood. The present work aimed to analyze the expression and activity of matrix metalloproteinases (MMPs), their endogenous inhibitors (TIMPs), and of Cn in patients with compensated LVH. By semiquantitative RT-PCR, Western blotting, and gelatine zymography, we determined mRNA, protein, and/or enzyme activity levels of MMPs, TIMPs, atrial natriuretic peptide (ANP), Cn subunits, and of the modulatory calcineurin-interacting protein (MCIP) 1. Myocardial samples from patients showing severe aortic stenosis, normal ejection fraction, and compensated LVH were compared with autopsy samples from healthy hearts. LVH patients showed upregulation of CnA-beta mRNA but downregulation of both CnB-alpha mRNA and protein. Total Cn activity (as determined through NF-AT phosphorylation and MCIP1 mRNA expression) was unchanged. There were no differences in gene expression and activities of MMP-2, MMP-9, and of TIMPs 1-4 between LVH patients and controls. As expected, ANP mRNA expression was high in LVH patients. We propose a prominent role for CnB in controlling Cn activity in compensated LVH. At this stage of the disease, MMP and TIMP activities are balanced.

RGD Manual Disease Annotations    Click to see Annotation Detail View

Objects Annotated

Genes (Rattus norvegicus)
Ppp3cb  (protein phosphatase 3 catalytic subunit beta)
Ppp3r1  (protein phosphatase 3, regulatory subunit B, alpha)

Genes (Mus musculus)
Ppp3cb  (protein phosphatase 3, catalytic subunit, beta isoform)
Ppp3r1  (protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I))

Genes (Homo sapiens)
PPP3CB  (protein phosphatase 3 catalytic subunit beta)
PPP3R1  (protein phosphatase 3 regulatory subunit B, alpha)


Additional Information