RGD Reference Report - Insulin receptor (IR) and glucose transporter 2 (GLUT2) proteins form a complex on the rat hepatocyte membrane. - Rat Genome Database

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Insulin receptor (IR) and glucose transporter 2 (GLUT2) proteins form a complex on the rat hepatocyte membrane.

Authors: Eisenberg, ML  Maker, AV  Slezak, LA  Nathan, JD  Sritharan, KC  Jena, BP  Geibel, JP  Andersen, DK 
Citation: Eisenberg ML, etal., Cell Physiol Biochem. 2005;15(1-4):51-8.
RGD ID: 1580591
Pubmed: PMID:15665515   (View Abstract at PubMed)
DOI: DOI:10.1159/000083638   (Journal Full-text)

The hepatic glucose transporter, GLUT2, facilitates bidirectional glucose transport across the hepatocyte plasma membrane under insulin regulation. We studied the interactions of IR and GLUT2 proteins to determine whether they are physically coupled in a receptor-transporter complex. By comparing endosome and plasma membrane IR and GLUT2 ratios before and after feeding, it was determined that IR and GLUT2 are internalized in a fixed ratio. When solubilized hepatocytes were immunoprecipitated with antibodies against either IR or GLUT2, both proteins co-precipitated. The association of IR and GLUT2 was further assessed by confocal microscopy. Sections of fed liver were incubated with fluorescein-tagged anti-GLUT2 or Texas Red-tagged anti-IR. Colocalization was observed both at the plasma membrane and in the cytosol. Fluorescence-resonance energy transfer studies further confirmed this association. We conclude that IR and GLUT2 form a receptor-transporter complex in hepatocytes, which forms a mechanism of insulin-mediated hepatic glucose regulation.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
endosome  IDA 1580591; 1580591 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
insulin receptor binding  IPIInsr (Rattus norvegicus)1580591 RGD 
protein binding  IPISlc2a2 (Rattus norvegicus)1580591 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Insr  (insulin receptor)
Slc2a2  (solute carrier family 2 member 2)


Additional Information