RGD Reference Report - Large-scale screen for genes controlling mammalian embryogenesis, using high-throughput gene expression analysis in mouse embryos. - Rat Genome Database

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Large-scale screen for genes controlling mammalian embryogenesis, using high-throughput gene expression analysis in mouse embryos.

Authors: Neidhardt, L  Gasca, S  Wertz, K  Obermayr, F  Worpenberg, S  Lehrach, H  Herrmann, BG 
Citation: Neidhardt L, etal., Mech Dev 2000 Nov;98(1-2):77-94.
RGD ID: 1556709
Pubmed: PMID:11044609   (View Abstract at PubMed)

We have adapted the whole-mount in situ hybridization technique to perform high-throughput gene expression analysis in mouse embryos. A large-scale screen for genes showing specific expression patterns in the mid-gestation embryo was carried out, and a large number of genes controlling development were isolated. From 35760 clones of a 9.5 d.p.c. cDNA library, a total of 5348 cDNAs, enriched for rare transcripts, were selected and analyzed by whole-mount in situ hybridization. Four hundred and twenty-eight clones revealed specific expression patterns in the 9.5 d.p.c. embryo. Of 361 tag-sequenced clones, 198 (55%) represent 154 known mouse genes. Thirty-nine (25%) of the known genes are involved in transcriptional regulation and 33 (21%) in inter- or intracellular signaling. A large number of these genes have been shown to play an important role in embryogenesis. Furthermore, 24 (16%) of the known genes are implicated in human disorders and three others altered in classical mouse mutations. Similar proportions of regulators of embryonic development and candidates for human disorders or mouse mutations are expected among the 163 new mouse genes isolated. Thus, high-throughput gene expression analysis is suitable for isolating regulators of embryonic development on a large-scale, and in the long term, for determining the molecular anatomy of the mouse embryo. This knowledge will provide a basis for the systematic investigation of pattern formation, tissue differentiation and organogenesis in mammals.

Objects referenced in this article
Gene Acp5 acid phosphatase 5, tartrate resistant Mus musculus
Gene Alas2 aminolevulinic acid synthase 2, erythroid Mus musculus
Gene Anxa2 annexin A2 Mus musculus
Gene Anxa3 annexin A3 Mus musculus
Gene Arg1 arginase, liver Mus musculus
Gene Arl4a ADP-ribosylation factor-like 4A Mus musculus
Gene Atp6v1e1 ATPase, H+ transporting, lysosomal V1 subunit E1 Mus musculus
Gene Bin1 bridging integrator 1 Mus musculus
Gene Bmp1 bone morphogenetic protein 1 Mus musculus
Gene Bnip3 BCL2/adenovirus E1B interacting protein 3 Mus musculus
Gene Bnip3l BCL2/adenovirus E1B interacting protein 3-like Mus musculus
Gene Cacna1g calcium channel, voltage-dependent, T type, alpha 1G subunit Mus musculus
Gene Cacnb3 calcium channel, voltage-dependent, beta 3 subunit Mus musculus
Gene Capn6 calpain 6 Mus musculus
Gene Car4 carbonic anhydrase 4 Mus musculus
Gene Ccnd2 cyclin D2 Mus musculus
Gene Cfl2 cofilin 2, muscle Mus musculus
Gene Cldn4 claudin 4 Mus musculus
Gene Cldn6 claudin 6 Mus musculus
Gene Cnn1 calponin 1 Mus musculus
Gene Col5a1 collagen, type V, alpha 1 Mus musculus
Gene Crabp2 cellular retinoic acid binding protein II Mus musculus
Gene Csrp1 cysteine and glycine-rich protein 1 Mus musculus
Gene Csrp2 cysteine and glycine-rich protein 2 Mus musculus
Gene Csrp3 cysteine and glycine-rich protein 3 Mus musculus
Gene Ctsl cathepsin L Mus musculus
Gene Cxcl12 C-X-C motif chemokine ligand 12 Mus musculus
Gene Cyp1b1 cytochrome P450, family 1, subfamily b, polypeptide 1 Mus musculus
Gene Dlk1 delta like non-canonical Notch ligand 1 Mus musculus
Gene Dll3 delta like canonical Notch ligand 3 Mus musculus
Gene Ebf1 early B cell factor 1 Mus musculus
Gene Emb embigin Mus musculus
Gene Epha4 Eph receptor A4 Mus musculus
Gene Epha7 Eph receptor A7 Mus musculus
Gene Flnc filamin C, gamma Mus musculus
Gene Fmod fibromodulin Mus musculus
Gene Gaa glucosidase, alpha, acid Mus musculus
Gene Gfi1b growth factor independent 1B Mus musculus
Gene Gja4 gap junction protein, alpha 4 Mus musculus
Gene Gpx1 glutathione peroxidase 1 Mus musculus
Gene Gpx3 glutathione peroxidase 3 Mus musculus
Gene Hand1 heart and neural crest derivatives expressed 1 Mus musculus
Gene Hbb-y hemoglobin Y, beta-like embryonic chain Mus musculus
Gene Hdac6 histone deacetylase 6 Mus musculus
Gene Hoxd10 homeobox D10 Mus musculus
Gene Hoxd3 homeobox D3 Mus musculus
Gene Hspb1 heat shock protein 1 Mus musculus
Gene Id1 inhibitor of DNA binding 1, HLH protein Mus musculus
Gene Id3 inhibitor of DNA binding 3 Mus musculus
Gene Ier2 immediate early response 2 Mus musculus
Gene Ifrd1 interferon-related developmental regulator 1 Mus musculus
Gene Itm2b integral membrane protein 2B Mus musculus
Gene Krt18 keratin 18 Mus musculus
Gene Krt8 keratin 8 Mus musculus
Gene Lamp2 lysosomal-associated membrane protein 2 Mus musculus
Gene Ldhb lactate dehydrogenase B Mus musculus
Gene Lmo4 LIM domain only 4 Mus musculus
Gene Lrig1 leucine-rich repeats and immunoglobulin-like domains 1 Mus musculus
Gene Lyl1 lymphoblastomic leukemia 1 Mus musculus
Gene Mcm3 minichromosome maintenance complex component 3 Mus musculus
Gene Meis2 Meis homeobox 2 Mus musculus
Gene Msi1 musashi RNA-binding protein 1 Mus musculus
Gene Msx3 msh homeobox 3 Mus musculus
Gene Myl1 myosin, light polypeptide 1 Mus musculus
Gene Myl7 myosin, light polypeptide 7, regulatory Mus musculus
Gene Myo5b myosin VB Mus musculus
Gene Nid2 nidogen 2 Mus musculus
Gene Nme1 NME/NM23 nucleoside diphosphate kinase 1 Mus musculus
Gene Nr2f6 nuclear receptor subfamily 2, group F, member 6 Mus musculus
Gene Nrp2 neuropilin 2 Mus musculus
Gene Pax9 paired box 9 Mus musculus
Gene Pfkm phosphofructokinase, muscle Mus musculus
Gene Plin2 perilipin 2 Mus musculus
Gene Psma2 proteasome subunit alpha 2 Mus musculus
Gene Ptgis prostaglandin I2 (prostacyclin) synthase Mus musculus
Gene Rbp1 retinol binding protein 1, cellular Mus musculus
Gene Rfx2 regulatory factor X, 2 (influences HLA class II expression) Mus musculus
Gene Rgn regucalcin Mus musculus
Gene Rpl13a ribosomal protein L13A Mus musculus
Gene Runx1t1 RUNX1 translocation partner 1 Mus musculus
Gene Selenop selenoprotein P Mus musculus
Gene Slc2a3 solute carrier family 2 (facilitated glucose transporter), member 3 Mus musculus
Gene Slit3 slit guidance ligand 3 Mus musculus
Gene Snai1 snail family zinc finger 1 Mus musculus
Gene Stc1 stanniocalcin 1 Mus musculus
Gene Tagln transgelin Mus musculus
Gene Tbx3 T-box 3 Mus musculus
Gene Tbx4 T-box 4 Mus musculus
Gene Tbx6 T-box 6 Mus musculus
Gene Tcf7l1 transcription factor 7 like 1 (T cell specific, HMG box) Mus musculus
Gene Tgfb1i1 transforming growth factor beta 1 induced transcript 1 Mus musculus
Gene Tnnc1 troponin C, cardiac/slow skeletal Mus musculus
Gene Tnni1 troponin I, skeletal, slow 1 Mus musculus
Gene Tnni2 troponin I, skeletal, fast 2 Mus musculus
Gene Tnni3 troponin I, cardiac 3 Mus musculus
Gene Tnnt2 troponin T2, cardiac Mus musculus
Gene Tpm2 tropomyosin 2, beta Mus musculus
Gene Ttr transthyretin Mus musculus
Gene Uncx UNC homeobox Mus musculus
Gene Urod uroporphyrinogen decarboxylase Mus musculus
Gene Utrn utrophin Mus musculus
Gene Vasp vasodilator-stimulated phosphoprotein Mus musculus
Gene Wnt5b wingless-type MMTV integration site family, member 5B Mus musculus
Gene Wnt7a wingless-type MMTV integration site family, member 7A Mus musculus
Gene Wnt7b wingless-type MMTV integration site family, member 7B Mus musculus

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