RGD Reference Report - Cloning and functional characterization of a rat Daxx that functions as a corepressor for the androgen receptor. - Rat Genome Database

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Cloning and functional characterization of a rat Daxx that functions as a corepressor for the androgen receptor.

Authors: Mizuta, H  Kuroda, Y 
Citation: Mizuta H and Kuroda Y, Cell Biol Int 2004;28(8-9):609-14.
RGD ID: 1358129
Pubmed: PMID:15350595   (View Abstract at PubMed)
DOI: DOI:10.1016/j.cellbi.2004.05.006   (Journal Full-text)

The androgen receptor (AR), a ligand-activated nuclear transcription factor, plays an important role in endocrine system development and homeostasis. AR-mediated gene transcription is modulated by a wide variety of coregulator proteins, but corepressors of AR have not been well characterized. We have isolated a rat homologue of Daxx, a possible AR repressor. The clone obtained comprised a 2196 bp open reading frame, corresponding to a sequence of 731 amino acids with 80.5% homology to mouse. Basal transcription of the isolated clone was repressed in transient transcription experiments using a Gal4 reporter gene assay. Furthermore, a mammalian two-hybrid assay showed a strong interaction between Daxx and promyelocytic leukemia (PML) protein. These results indicate that the isolated clone is a rat homologue of Daxx. The rat Daxx (rDaxx) interacted with the androgen receptor (AR): in co-immunoprecipitation experiments, AR formed complexes with anti-Daxx antibody. Furthermore, overexpression of Daxx suppressed AR-mediated transcriptional activity in HeLa and CV1 cells in transient transfection experiments. Taken together, our results suggest that Daxx may function as a corepressor for the androgen receptor.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of transcription by RNA polymerase II  IDA 1358129 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
nuclear androgen receptor binding  IPIAr (Rattus norvegicus)1358129 RGD 
protein binding  IPIDaxx (Rattus norvegicus)1358129 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ar  (androgen receptor)
Daxx  (death-domain associated protein)


Additional Information