RGD Reference Report - CAPS drives trans-SNARE complex formation and membrane fusion through syntaxin interactions. - Rat Genome Database

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CAPS drives trans-SNARE complex formation and membrane fusion through syntaxin interactions.

Authors: James, Declan J  Kowalchyk, Judith  Daily, Neil  Petrie, Matt  Martin, Thomas F J 
Citation: James DJ, etal., Proc Natl Acad Sci U S A. 2009 Oct 13;106(41):17308-13. doi: 10.1073/pnas.0900755106. Epub 2009 Sep 29.
RGD ID: 13432245
Pubmed: PMID:19805029   (View Abstract at PubMed)
PMCID: PMC2765074   (View Article at PubMed Central)
DOI: DOI:10.1073/pnas.0900755106   (Journal Full-text)

Ca(2+)-dependent activator protein for secretion (CAPS) is an essential factor for regulated vesicle exocytosis that functions in priming reactions before Ca(2+)-triggered fusion of vesicles with the plasma membrane. However, the precise events that CAPS regulates to promote vesicle fusion are unclear. In the current work, we reconstituted CAPS function in a SNARE-dependent liposome fusion assay using VAMP2-containing donor and syntaxin-1/SNAP-25-containing acceptor liposomes. The CAPS stimulation of fusion required PI(4,5)P(2) in acceptor liposomes and was independent of Ca(2+), but Ca(2+) dependence was restored by inclusion of synaptotagmin. CAPS stimulated trans-SNARE complex formation concomitant with the stimulation of full membrane fusion at physiological SNARE densities. CAPS bound syntaxin-1, and CAPS truncations that competitively inhibited syntaxin-1 binding also inhibited CAPS-dependent fusion. The results revealed an unexpected activity of a priming protein to accelerate fusion by efficiently promoting trans-SNARE complex formation. CAPS may function in priming by organizing SNARE complexes on the plasma membrane.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding enablesIPIUniProtKB:P3285113432245PMID:19805029IntAct 
protein binding enablesIPIUniProtKB:P6087913432245PMID:19805029IntAct 
protein binding enablesIPIUniProtKB:Q6271713432245PMID:19805029IntAct 

Objects Annotated

Genes (Rattus norvegicus)
Cadps  (calcium dependent secretion activator)
Stx1a  (syntaxin 1A)


Additional Information