RGD Reference Report - Purification and identification of secreted oxidative stress-induced factors from vascular smooth muscle cells. - Rat Genome Database

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Purification and identification of secreted oxidative stress-induced factors from vascular smooth muscle cells.

Authors: Liao, DF  Jin, ZG  Baas, AS  Daum, G  Gygi, SP  Aebersold, R  Berk, BC 
Citation: Liao DF, etal., J Biol Chem 2000 Jan 7;275(1):189-96.
RGD ID: 1304537
Pubmed: PMID:10617604   (View Abstract at PubMed)

Reactive oxygen species have been implicated in the pathogenesis of atherosclerosis and hypertension, in part by promoting vascular smooth muscle cell (VSMC) growth. We have previously shown that LY83583, a generator of O-(2), activated extracellular signal-regulated kinases (ERK1/2) with early (10 min) and late (2 h) peaks and stimulated VSMC growth. To investigate whether secreted oxidative stress-induced factors (termed SOXF) from VSMC were responsible for late ERK1/2 activation in response to LY83583, we purified putative SOXF proteins from conditioned medium (2 h of LY83583 exposure) by sequential chromatography based on activation of ERK1/2. Proteins identified by capillary chromatography, electrospray ionization tandem mass spectrometry, and data base searching included heat shock protein 90-alpha (HSP90-alpha) and cyclophilin B. Western blot analysis of conditioned medium showed specific secretion of HSP90-alpha but not HSP90-beta. Immunodepletion of HSP90-alpha from conditioned medium significantly inhibited conditioned medium-induced ERK1/2 activation. Human recombinant HSP90-alpha reproduced the effect of conditioned medium on ERK1/2 activation. These results show that brief oxidative stress causes sustained release of protein factors from VSMC that can stimulate ERK1/2. These factors may be important mediators for the effects of reactive oxygen species on vascular function.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to organic cyclic compound  IEP 1304537guanylate cyclase inhibitor LY83583RGD 
protein folding  TAS 1304537 RGD 
response to unfolded protein  TAS 1304537 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
ATP binding  TAS 1304537 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hsp90ab1  (heat shock protein 90 alpha family class B member 1)


Additional Information