RGD Reference Report - Beta-dystrobrevin interacts directly with kinesin heavy chain in brain. - Rat Genome Database

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Beta-dystrobrevin interacts directly with kinesin heavy chain in brain.

Authors: Macioce, P  Gambara, G  Bernassola, M  Gaddini, L  Torreri, P  Macchia, G  Ramoni, C  Ceccarini, M  Petrucci, TC 
Citation: Macioce P, etal., J Cell Sci 2003 Dec 1;116(Pt 23):4847-56.
RGD ID: 1304517
Pubmed: PMID:14600269   (View Abstract at PubMed)
DOI: DOI:10.1242/jcs.00805   (Journal Full-text)

Beta-dystrobrevin, a member of the dystrobrevin protein family, is a dystrophin-related and -associated protein restricted to non-muscle tissues and is highly expressed in kidney, liver and brain. Dystrobrevins are now thought to play an important role in intracellular signal transduction, in addition to providing a membrane scaffold in muscle, but the precise role of beta-dystrobrevin has not yet been determined. To study beta-dystrobrevin's function in brain, we used the yeast two-hybrid approach to look for interacting proteins. Four overlapping clones were identified that encoded Kif5A, a neuronal member of the Kif5 family of proteins that consists of the heavy chains of conventional kinesin. A direct interaction of beta-dystrobrevin with Kif5A was confirmed by in vitro and in vivo association assays. Co-immunoprecipitation with a monoclonal kinesin heavy chain antibody precipitated both alpha- and beta-dystrobrevin, indicating that this interaction is not restricted to the beta-dystrobrevin isoform. The site for Kif5A binding to beta-dystrobrevin was localized in a carboxyl-terminal region that seems to be important in heavy chain-mediated kinesin interactions and is highly homologous in all three Kif5 isoforms, Kif5A, Kif5B and Kif5C. Pull-down and immunofluorescence experiments also showed a direct interaction between beta-dystrobrevin and Kif5B. Our findings suggest a novel function for dystrobrevin as a motor protein receptor that might play a major role in the transport of components of the dystrophin-associated protein complex to specific sites in the cell.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
microtubule-based process  TAS 1304517 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
kinesin complex  TAS 1304517 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
microtubule motor activity  TAS 1304517 RGD 
protein binding enablesIPIUniProtKB:Q6QLM71304517PMID:14600269UniProt 
protein binding enablesIPIUniProtKB:P840601304517PMID:14600269UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Dtnb  (dystrobrevin, beta)
Kif5a  (kinesin family member 5A)

Objects referenced in this article
Gene Dtnb dystrobrevin, beta Mus musculus
Gene Kif5a kinesin family member 5A Mus musculus

Additional Information