RGD Reference Report - Src-dependent association of Cas and p85 phosphatidylinositol 3'-kinase in v-crk-transformed cells. - Rat Genome Database

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Src-dependent association of Cas and p85 phosphatidylinositol 3'-kinase in v-crk-transformed cells.

Authors: Riggins, RB  DeBerry, RM  Toosarvandani, MD  Bouton, AH 
Citation: Riggins RB, etal., Mol Cancer Res 2003 Apr;1(6):428-37.
RGD ID: 1304375
Pubmed: PMID:12692262   (View Abstract at PubMed)

Cellular changes associated with oncogenic transformation are generally caused by deregulation of signal transduction pathways. We show that, in cells transformed by the v-crk oncogene, the adapter protein Cas forms a stable complex with the p85 regulatory subunit of phosphatidylinositol 3'-kinase (PI3K) coincident with the appearance of Cas-associated PI3K activity. The interaction between Cas and p85 PI3K appears to be driven primarily by Src-dependent tyrosine phosphorylation of Cas, and mapping studies indicate that the carboxyl terminus of Cas is necessary and sufficient for binding to p85 PI3K. One of the cellular effects of v-Crk expression is to promote DNA synthesis in the presence of low serum. This effect is potentiated in Cas-null fibroblasts when wild-type Cas is expressed, but not when a Cas variant is expressed that lacks the carboxyl-terminal p85 PI3K binding region. This suggests that the association of Cas with p85 PI3K may play a role in uncoupling growth regulatory pathways through v-Crk.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding  IPIPik3r1 (Rattus norvegicus)1304375 RGD 
protein binding  IPIBcar1 (Rattus norvegicus)1304375protein C-terminus bindingRGD 

Objects Annotated

Genes (Rattus norvegicus)
Bcar1  (BCAR1 scaffold protein, Cas family member)
Pik3r1  (phosphoinositide-3-kinase regulatory subunit 1)


Additional Information