RGD Reference Report - The PDZ-LIM protein RIL modulates actin stress fiber turnover and enhances the association of alpha-actinin with F-actin. - Rat Genome Database

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The PDZ-LIM protein RIL modulates actin stress fiber turnover and enhances the association of alpha-actinin with F-actin.

Authors: Vallenius, T  Scharm, B  Vesikansa, A  Luukko, K  Schafer, R  Makela, TP 
Citation: Vallenius T, etal., Exp Cell Res 2004 Feb 1;293(1):117-28.
RGD ID: 1300470
Pubmed: PMID:14729062   (View Abstract at PubMed)

ALP, CLP-36 and RIL form the ALP subfamily of PDZ-LIM proteins. ALP has been implicated in sarcomere function in muscle cells in association with alpha-actinin. The closely related CLP-36 is predominantly expressed in nonmuscle cells, where it localizes to actin stress fibers also in association with alpha-actinin. Here we have studied the expression and functions of RIL originally identified as a gene downregulated in H-ras-transformed cells. RIL was mostly expressed in nonmuscle epithelial cells with a pattern distinct from that of CLP-36. RIL protein was found to localize to actin stress fibers in nonmuscle cells similarly to CLP-36. However, RIL expression led to partially abnormal actin filaments showing thick irregular stress fibers not seen with CLP-36. Furthermore, live cell imaging demonstrated altered stress fiber dynamics with rapid formation of new fibers and frequent collapse of thick irregular fibers in EGFP-RIL-expressing cells. These effects may be mediated through the association of RIL with alpha-actinin, as RIL was found to associate with alpha-actinin via its PDZ domain, and RIL enhanced the ability of alpha-actinin to cosediment with actin filaments. These results implicate the RIL PDZ-LIM protein as a regulator of actin stress fiber turnover.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
actin cytoskeleton organization  IDA 1300470 RGD 
positive regulation of stress fiber assembly involved_inIDA 1300470PMID:14729062UniProt 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
actin cytoskeleton located_inIPIUniProtKB:Q9Z1P21300470PMID:14729062UniProt 
stress fiber located_inIDA 1300470PMID:14729062UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
actinin binding  IPIUniProtKB:P050941300470 RGD 
alpha-actinin binding enablesIPIUniProtKB:Q9Z1P21300470PMID:14729062UniProt 
protein binding enablesIPIUniProtKB:P362021300470PMID:14729062UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Actn1  (actinin, alpha 1)
Pdlim4  (PDZ and LIM domain 4)

Objects referenced in this article
Gene Ldb2 LIM domain binding 2 Mus musculus
Gene Pdlim3 PDZ and LIM domain 3 Mus musculus

Additional Information