RGD Reference Report - SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis. - Rat Genome Database

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SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis.

Authors: Chin, LS  Nugent, RD  Raynor, MC  Vavalle, JP  Li, L 
Citation: Chin LS, etal., J Biol Chem 2000 Jan 14;275(2):1191-200.
RGD ID: 1299445
Pubmed: PMID:10625663   (View Abstract at PubMed)

Synaptosome-associated protein of 25 kDa (SNAP-25) is a presynaptic membrane protein that has been clearly implicated in membrane fusion in both developing and mature neurons, although its mechanisms of action are unclear. We have now identified a novel SNAP-25-interacting protein named SNIP. SNIP is a hydrophilic, 145-kDa protein that comprises two predicted coiled-coil domains, two highly charged regions, and two proline-rich domains with multiple PPXY and PXXP motifs. SNIP is selectively expressed in brain where it co-distributes with SNAP-25 in most brain regions. Biochemical studies have revealed that SNIP is tightly associated with the brain cytoskeleton. Subcellular fractionation and immunofluorescence localization studies have demonstrated that SNIP co-localizes with SNAP-25 as well as the cortical actin cytoskeleton, suggesting that SNIP serves as a linker protein connecting SNAP-25 to the submembranous cytoskeleton. By using deletion analysis, we have mapped the binding domains of SNIP and SNAP-25, and we have demonstrated that the SNIP-SNAP-25 association is mediated via coiled-coil interactions. Moreover, we have shown that overexpression of SNIP or its SNAP-25-interacting domain inhibits Ca(2+)-dependent exocytosis from PC12 cells. These results indicate that SNIP is involved in regulation of neurosecretion, perhaps via its interaction with SNAP-25 and the cytoskeleton.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of protein secretion  IMP 1299445 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
actin cytoskeleton  IDA 1299445; 1299445 RGD 
filopodium  IDA 1299445; 1299445 RGD 
lamellipodium  IDA 1299445; 1299445 RGD 
neuronal cell body  IDA 1299445; 1299445 RGD 

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Snap25  (synaptosome associated protein 25)
Srcin1  (SRC kinase signaling inhibitor 1)


Additional Information