RGD Reference Report - Nexilin: a novel actin filament-binding protein localized at cell-matrix adherens junction. - Rat Genome Database

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Nexilin: a novel actin filament-binding protein localized at cell-matrix adherens junction.

Authors: Ohtsuka, T  Nakanishi, H  Ikeda, W  Satoh, A  Momose, Y  Nishioka, H  Takai, Y 
Citation: Ohtsuka T, etal., J Cell Biol 1998 Nov 30;143(5):1227-38.
RGD ID: 1299387
Pubmed: PMID:9832551   (View Abstract at PubMed)
PMCID: PMC2133087   (View Article at PubMed Central)

We isolated two novel actin filament (F-actin)-binding proteins from rat brain and rat 3Y1 fibroblast. They were splicing variants, and we named brain big one b-nexilin and fibroblast small one s-nexilin. b-Nexilin purified from rat brain was a protein of 656 amino acids (aa) with a calculated molecular weight of 78,392, whereas s-nexilin, encoded by the cDNA isolated from rat 3Y1 cells by the reverse transcriptase-PCR method, was a protein of 606 aa with a calculated molecular weight of 71,942. b-Nexilin had two F-actin- binding domains (ABDs) at the NH2-terminal and middle regions, whereas s-nexilin had one ABD at the middle region because 64 aa residues were deleted and 14 aa residues were inserted in the first NH2-terminal ABD of b-nexilin, and thereby the first ABD lost its activity. b- and s-nexilins bound along the sides of F-actin, but only b-nexilin showed F-actin cross-linking activity. b-Nexilin was mainly expressed in brain and testis, whereas s-nexilin was mainly expressed in testis, spleen, and fibroblasts, such as rat 3Y1 and mouse Swiss 3T3 cells, but neither b- nor s-nexilin was detected in liver, kidney, or cultured epithelial cells. An immunofluorescence microscopic study revealed that s-nexilin was colocalized with vinculin, talin, and paxillin at cell- matrix adherens junction (AJ) and focal contacts, but not at cell-cell AJ, in 3Y1 cells. Overexpressed b- and s-nexilins were localized at focal contacts but not at cell-cell AJ. These results indicate that nexilin is a novel F-actin-binding protein localized at cell-matrix AJ.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cell-substrate junction  IDA 1299387 RGD 
focal adhesion located_inIDA 1299387PMID:9832551UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
actin filament binding  IDA 1299387 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nexn  (nexilin (F actin binding protein))


Additional Information