RGD Reference Report - Molecular cloning and nucleotide sequence of a cDNA clone coding for rat brain myelin proteolipid. - Rat Genome Database

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Molecular cloning and nucleotide sequence of a cDNA clone coding for rat brain myelin proteolipid.

Authors: Dautigny, A  Alliel, PM  D'Auriol, L  Pham Dinh, D  Nussbaum, JL  Galibert, F  Jolles, P 
Citation: Dautigny A, etal., FEBS Lett 1985 Aug 19;188(1):33-6.
RGD ID: 1298601
Pubmed: PMID:2410294   (View Abstract at PubMed)

A cDNA library from rat brain was constructed in pBR322 and screened with a 14-mer mixed oligonucleotide probe based on residues 231-235 of bovine proteolipid (PLP). A positive clone was isolated: it contained a 1334-base-pair cDNA insert and was subjected to DNA sequence analysis. The cDNA encoded information for the 276 amino acids of rat PLP. Comparison with bovine PLP sequence showed a complete amino acid sequence homology except for 4 amino acid residues.

Objects referenced in this article
Gene Plp1 proteolipid protein 1 Rattus norvegicus

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