RGD Reference Report - Upregulation of cardiovascular ghrelin receptor occurs in the hyperdynamic phase of sepsis. - Rat Genome Database

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Upregulation of cardiovascular ghrelin receptor occurs in the hyperdynamic phase of sepsis.

Authors: Wu, Rongqian  Zhou, Mian  Cui, Xiaoxuan  Simms, H Hank  Wang, Ping 
Citation: Wu R, etal., Am J Physiol Heart Circ Physiol. 2004 Sep;287(3):H1296-302. Epub 2004 May 20.
RGD ID: 12905047
Pubmed: PMID:15155262   (View Abstract at PubMed)
DOI: DOI:10.1152/ajpheart.00852.2003   (Journal Full-text)

Ghrelin, a newly identified endogenous ligand for growth hormone secretagogue receptor 1a (GHSR-1a, i.e., ghrelin receptor), was recently demonstrated to be a potent vasoactive peptide. Although sepsis is characterized by an early, hyperdynamic phase, it remains unknown whether ghrelin or GHSR-1a plays a role in the cardiovascular response to sepsis. To determine this, polymicrobial sepsis was induced by cecal ligation and puncture in male adult rats. At 5 h (i.e., early sepsis) or 20 h (i.e., late sepsis) after cecal ligation and puncture, blood and tissue samples were collected. Ghrelin levels and ghrelin and GHSR-1a mRNA expression were assessed by RIA and RT-PCR, respectively. In addition, GHSR-1a protein levels in aorta, heart, and small intestine were determined by Western blotting. The vascular response to ghrelin was determined by using an isolated gut preparation. A primary rat aortic smooth muscle cell culture was used to determine the effects of LPS on GHSR-1a expression. The results indicate that although ghrelin levels decreased at early and late sepsis, its receptor was markedly elevated in early sepsis. Moreover, ghrelin-induced relaxation in resistance blood vessels of the isolated small intestine increased significantly during early sepsis but was not altered in late sepsis. Furthermore, GHSR-1a expression in smooth muscle cells was significantly increased at mRNA and protein levels with stimulation by LPS at 10 ng/ml. These results demonstrate that GHSR-1a expression is upregulated and vascular sensitivity to ghrelin stimulation is increased in the hyperdynamic phase of sepsis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Sepsis  ISOGhrl (Rattus norvegicus)12905047; 12905047mRNA more ...RGD 
Sepsis disease_progressionISOGhsr (Rattus norvegicus)12905047; 12905047 RGD 
Sepsis  IEP 12905047mRNA more ...RGD 
Sepsis disease_progressionIEP 12905047 RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to lipopolysaccharide  IEP 12905047 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ghrl  (ghrelin and obestatin prepropeptide)
Ghsr  (growth hormone secretagogue receptor)

Genes (Mus musculus)
Ghrl  (ghrelin)
Ghsr  (growth hormone secretagogue receptor)

Genes (Homo sapiens)
GHRL  (ghrelin and obestatin prepropeptide)
GHSR  (growth hormone secretagogue receptor)


Additional Information