RGD Reference Report - Delta-aminolevulinate dehydratase activity in red blood cells of rats infected with Trypanosoma evansi. - Rat Genome Database

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Delta-aminolevulinate dehydratase activity in red blood cells of rats infected with Trypanosoma evansi.

Authors: França, Raqueli T  Da Silva, Aleksandro S  Wolkmer, Patrícia  Oliveira, Vitor A  Pereira, Maria E  Leal, Marta L R  Silva, Cássia B  Nunes, Matheus A G  Dressler, Valderi L  Mazzanti, Cinthia M  Monteiro, Silvia G  Lopes, Sonia T A 
Citation: França RT, etal., Parasitology. 2011 Sep;138(10):1272-7. doi: 10.1017/S0031182011000989.
RGD ID: 12904694
Pubmed: PMID:21854703   (View Abstract at PubMed)
DOI: DOI:10.1017/S0031182011000989   (Journal Full-text)

The aim of this study was to evaluate the activity of delta-aminolevulinate dehydratase (d-ALA-D) in red blood cells of rats infected with Trypanosoma evansi and establish its association with haematocrit, serum levels of iron and zinc and lipid peroxidation. Thirty-six male rats (Wistar) were divided into 2 groups with 18 animals each. Group A was non-infected while Group B was intraperitoneally infected, receiving 7·5×106 trypomastigotes per animal. Each group was divided into 3 subgroups of 6 rats and blood was collected during different periods post-infection (p.i.) as follows: day 5 (A1 and B1), day 15 (A2 and B2) and day 30 PI (A3 and B3). Blood samples were collected by cardiac puncture to estimate red blood cell parameters (RBC), d-ALA-D activity and serum levels of iron, zinc and thiobarbituric acid reactive substances (TBARS). Rats in group B showed a significant (P<0·05) reduction of RBC count, haemoglobin concentration and haematocrit at days 5 and 15 p.i. The activity of d-ALA-D in blood was significantly (P<0·001) increased at days 15 and 30 p.i. d-ALA-D activity in blood had a significant (P<0·05) negative correlation with haematocrit (r=-0·61) and haemoglobin (r=-0·70) at day 15 p.i. There was a significant (P<0·05) decrease in serum iron and zinc levels and an increase in TBARS levels (P<0·05) during infection. The d-ALA-D activity in blood was negatively correlated with the levels of iron (r=-0·68) and zinc (r=-0·57) on day 30 p.i. It was concluded that the increased activity of d-ALA-D in blood might have occurred in response to the anaemia in remission as heme synthesis was enhanced.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
anemia  ISOAlad (Rattus norvegicus)12904694; 12904694associated with Trypanosomiasis and protein:increased activity:erythrocyteRGD 
anemia  IDA 12904694associated with Trypanosomiasis and protein:increased activity:erythrocyteRGD 

Objects Annotated

Genes (Rattus norvegicus)
Alad  (aminolevulinate dehydratase)

Genes (Mus musculus)
Alad  (aminolevulinate, delta-, dehydratase)

Genes (Homo sapiens)
ALAD  (aminolevulinate dehydratase)


Additional Information