RGD Reference Report - Metallothionein treatment reduces proinflammatory cytokines IL-6 and TNF-alpha and apoptotic cell death during experimental autoimmune encephalomyelitis (EAE).

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Metallothionein treatment reduces proinflammatory cytokines IL-6 and TNF-alpha and apoptotic cell death during experimental autoimmune encephalomyelitis (EAE).
Authors:	Penkowa, M Hidalgo, J
Citation:	Penkowa M and Hidalgo J, Exp Neurol. 2001 Jul;170(1):1-14.
RGD ID:	12904661
Pubmed:	PMID:11421579 (View Abstract at PubMed)
DOI:	DOI:10.1006/exnr.2001.7675 (Journal Full-text)
Experimental autoimmune encephalomyelitis (EAE) is an animal model for the human autoimmune disease multiple sclerosis (MS). Proinflammatory cytokines such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) are considered important for induction and pathogenesis of EAE/MS disease, which is characterized by significant inflammation and neuroglial damage. We have recently shown that the exogenous administration of the antioxidant protein zinc-metallothionein-II (Zn-MT-II) significantly decreased the clinical symptoms, mortality, and leukocyte infiltration of the CNS during EAE. However, it is not known how EAE progression is regulated nor how cytokine production and cell death can be reduced. We herewith demonstrate that treatment with Zn-MT-II significantly decreased the CNS expression of IL-6 and TNF-alpha during EAE. Zn-MT-II treatment could also significantly reduce apoptotic cell death of neurons and oligodendrocytes during EAE, as judged by using TUNEL and immunoreactivity for cytochrome c and caspases 1 and 3. In contrast, the number of apoptotic lymphocytes and macrophages was less affected by Zn-MT-II treatment. The Zn-MT-II-induced decrease in proinflammatory cytokines and apoptosis during EAE could contribute to the reported diminution of clinical symptoms and mortality in EAE-immunized rats receiving Zn-MT-II treatment. Our results demonstrate that MT-II reduces the CNS expression of proinflammatory cytokines and the number of apoptotic neurons during EAE in vivo and that MT-II might be a potentially useful factor for treatment of EAE/MS.

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
Experimental Autoimmune Encephalomyelitis	treatment	ISO	Tnf (Rattus norvegicus)	12904661; 12904661		RGD
Experimental Autoimmune Encephalomyelitis	treatment	IEP		12904661		RGD

Objects Annotated

Genes (Rattus norvegicus)

Tnf (tumor necrosis factor)

Genes (Mus musculus)

Tnf (tumor necrosis factor)

Genes (Homo sapiens)

TNF (tumor necrosis factor)

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Metallothionein treatment reduces proinflammatory cytokines IL-6 and TNF-alpha and apoptotic cell death during experimental autoimmune encephalomyelitis (EAE).