RGD Reference Report - Sonic hedgehog expression in a rat model of chronic pancreatitis. - Rat Genome Database

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Sonic hedgehog expression in a rat model of chronic pancreatitis.

Authors: Wang, Luo-Wei  Lin, Han  Lu, Yi  Xia, Wei  Gao, Jun  Li, Zhao-Shen 
Citation: Wang LW, etal., World J Gastroenterol. 2014 Apr 28;20(16):4712-7. doi: 10.3748/wjg.v20.i16.4712.
RGD ID: 12879405
Pubmed: PMID:24782623   (View Abstract at PubMed)
PMCID: PMC4000507   (View Article at PubMed Central)
DOI: DOI:10.3748/wjg.v20.i16.4712   (Journal Full-text)


AIM: To analyze the activation of sonic hedgehog (SHh) signaling pathways in a rat model of chronic pancreatitis.
METHODS: Forty Wistar rats were randomly divided into 2 groups: experimental group and control group (20 rats in each group). Dibutyltin dichloride was infused into the tail vein of the rats to induce chronic pancreatitis in the experimental group. The same volume of ethanol and glycerol mixture was infused in the control group. The expression of Ptch, Smo and Gli were analyzed using immunohistochemistry, and real-time reverse transcription polymerase chain reaction (RT-PCR).
RESULTS: Compared with the control group, significant histological changes in terms of the areas of abnormal architecture, glandular atrophy, fibrosis, pseudo tubular complexes, and edema were observed at week 4 in the experimental group. The expression of Ptch1, Smo and Gli1 in the pancreatic tissue increased significantly in the experimental group. Using RT-PCR, mRNA levels of Ptch, Smo and Gli in the experimental group increased significantly compared with the control group.
CONCLUSION: The SHh signaling pathway is aberrantly activated in rats with chronic pancreatitis. The SHh signaling pathway plays an important role in the development of chronic pancreatitis. These results may be helpful in studies focusing on the relationship between chronic pancreatitis and pancreatic cancer.

RGD Manual Disease Annotations    Click to see Annotation Detail View

Objects Annotated

Genes (Rattus norvegicus)
Gli1  (GLI family zinc finger 1)
Ptch1  (patched 1)
Smo  (smoothened, frizzled class receptor)

Genes (Mus musculus)
Gli1  (GLI-Kruppel family member GLI1)
Ptch1  (patched 1)
Smo  (smoothened, frizzled class receptor)

Genes (Homo sapiens)
GLI1  (GLI family zinc finger 1)
PTCH1  (patched 1)
SMO  (smoothened, frizzled class receptor)


Additional Information