RGD Reference Report - Expression of Wnt pathway mediators in metaplasic tissue in animal model and clinical samples of tendinopathy. - Rat Genome Database

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Expression of Wnt pathway mediators in metaplasic tissue in animal model and clinical samples of tendinopathy.

Authors: Lui, Pauline Po Yee  Lee, Yuk Wa  Wong, Yin Mei  Zhang, Xiaoling  Dai, Kerong  Rolf, Christer Gustav 
Citation: Lui PP, etal., Rheumatology (Oxford). 2013 Sep;52(9):1609-18. doi: 10.1093/rheumatology/ket214. Epub 2013 Jun 17.
RGD ID: 12793064
Pubmed: PMID:23776285   (View Abstract at PubMed)
DOI: DOI:10.1093/rheumatology/ket214   (Journal Full-text)


OBJECTIVE: Tissue metaplasia is observed in both ossified failed healing animal model and clinical samples of tendinopathy. The Wnt signalling pathway plays a vital role in pathological calcification. We hypothesized that the Wnt signalling pathway might contribute to tissue metaplasia and failed healing in tendinopathy. This study aimed to examine the spatial-temporal expression of Wnt pathway mediators in an ossified failed tendon healing animal model and clinical samples of tendinopathy. The effect of Wnt3a on the osteogenic differentiation of tendon-derived stem cells (TDSCs) was also examined.
METHODS: Ossified failed tendon healing was induced by the injection of collagenase into the patellar tendon of rats. At various times the tendons were harvested for immunohistochemical staining of Wnt3a, ß-catenin, Lrp5 and Tcf1. Patellar tendon samples were obtained from 13 patients with patellar tendinopathy (11 unossified and 2 ossified) and 10 controls. Immunohistochemical staining of Wnt3a, ß-catenin, Lrp5 and Tcf1 was similarly performed. Rat patellar TDSCs were treated with Wnt3a. The osteogenic differentiation of TDSCs was examined by ALP activity, alizarin red S staining and mRNA expression of osteogenic markers.
RESULTS: There was increased expression of Wnt3a, ß-catenin, Lrp5 and Tcf1 in the healing fibroblast-like cells, chondrocyte-like cells and ossified deposits in the animal model and in some clinical samples of tendinopathy. Wnt3a increased ALP activity, calcium nodule formation and expression of osteogenic markers in TDSCs.
CONCLUSION: Activation of the Wnt signalling pathway and its effect on TDSCs might contribute to tissue metaplasia and failed healing in some cases of tendinopathy.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
tendinitis  ISOLrp5 (Rattus norvegicus)12793064; 12793064protein:increased expression:patellar tendon:RGD 
tendinitis  IEP 12793064protein:increased expression:patellar tendon:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Lrp5  (LDL receptor related protein 5)

Genes (Mus musculus)
Lrp5  (low density lipoprotein receptor-related protein 5)

Genes (Homo sapiens)
LRP5  (LDL receptor related protein 5)


Additional Information