RGD Reference Report - Proteomic characterisation of neuronal sphingolipid-cholesterol microdomains: role in plasminogen activation. - Rat Genome Database

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Proteomic characterisation of neuronal sphingolipid-cholesterol microdomains: role in plasminogen activation.

Authors: Ledesma, Maria Dolores  Da Silva, Jorge Santos  Schevchenko, Anna  Wilm, Matthias  Dotti, Carlos G 
Citation: Ledesma MD, etal., Brain Res. 2003 Oct 10;987(1):107-16.
RGD ID: 12793007
Pubmed: PMID:14499952   (View Abstract at PubMed)

Sorting of certain membrane proteins requires a mechanism involving rafts, protein-lipid complexes enriched in glycosphingolipids and cholesterol. These microdomains remain at the plasma membrane of different cell types and play a role in signal transduction. Although recent reports have begun to describe molecules associated with rafts, their protein composition remains largely unknown, especially in neuronal cells. To address this question, we have purified detergent-insoluble raft fractions (DRMs) from primary cultures of hippocampal neurons. Bidimensional gel analysis and pharmacological raft lipid manipulation allowed the identification of neuronal raft proteins and their characterisation by MALDI-TOF analysis. Enolases were found among the proteins identified and functional studies demonstrate their participation in plasminogen binding. We also show the specific enrichment in rafts of several other plasminogen binding molecules and the exclusive activation of plasminogen to the protease plasmin in these microdomains. These observations suggest that neuronal rafts may play, in addition to intracellular signaling, a role in extracellular/membrane protein proteolysis.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
enzyme binding enablesIPIUniProtKB:P0732312793007PMID:14499952CAFA 
enzyme binding enablesIPIUniProtKB:P0476412793007PMID:14499952CAFA 

Objects Annotated

Genes (Rattus norvegicus)
Actb  (actin, beta)
Anxa2  (annexin A2)
Atp5f1a  (ATP synthase F1 subunit alpha)
Atp5f1b  (ATP synthase F1 subunit beta)
Eno1  (enolase 1)
Eno2  (enolase 2)
Hmgb1  (high mobility group box 1)
Hspa1a  (heat shock protein family A (Hsp70) member 1A)
Hspa1b  (heat shock protein family A (Hsp70) member 1B)
Slc25a4  (solute carrier family 25 member 4)
Slc25a5  (solute carrier family 25 member 5)
Thy1  (Thy-1 cell surface antigen)
Tuba1a  (tubulin, alpha 1A)
Tuba1b  (tubulin, alpha 1B)
Tuba1c  (tubulin, alpha 1C)
Tubb5  (tubulin, beta 5 class I)


Additional Information