RGD Reference Report - Coordinated regulation and colocalization of alphav integrin and its activating enzyme proprotein convertase PC5 in vivo. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Coordinated regulation and colocalization of alphav integrin and its activating enzyme proprotein convertase PC5 in vivo.

Authors: Stawowy, P  Graf, K  Goetze, S  Roser, M  Chretien, M  Seidah, NG  Fleck, E  Marcinkiewicz, M 
Citation: Stawowy P, etal., Histochem Cell Biol. 2003 Mar;119(3):239-45. Epub 2003 Feb 20.
RGD ID: 11556213
Pubmed: PMID:12649739   (View Abstract at PubMed)
DOI: DOI:10.1007/s00418-003-0506-7   (Journal Full-text)

Integrin alphav is involved in intracellular-extracellular signaling important for cytoskeleton alterations and control of cell movement. In vitro experiments indicate that the integrin alphav-subunit undergoes post-translational endoproteolytic cleavage. This type of activation requires the presence of suitable kexin/subtilisin-like proprotein convertases. In vitro experiments have demonstrated that, among several proprotein convertases, PC5A, and to a threefold lesser extent furin, can activate alphav integrin. The biological significance of these in vitro data would be further supported by a coexpression and coordinated regulation of the gene expression of alphav integrin and its activating enzyme PC5 in vivo. In the present study we investigated the regulation of alphav integrin and PC5 following balloon injury in vivo. Comparative immunocytochemistry revealed a coordinated regulation of alphav integrin and PC5 during vascular remodeling in rodents. Integrin alphav was found to be upregulated in PCNA-positive, proliferating vascular smooth muscle cells. Northern blots revealed no significant regulation of furin mRNA, whereas PC5A mRNA increased during vascular remodeling, suggesting that PC5 is the major convertase during neointima formation in vivo. Incubation of vascular smooth muscle cells with the Golgi-disturbing agent brefeldin A inhibited alphav integrin maturation, indicating that endoproteolytic cleavage occurs in the trans-Golgi network, were PC5 is localized. Thus, the present study further supports the concept that activation of alphav integrin occurs in the trans-Golgi network in vascular smooth muscle cells and involves PC5.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Carotid Artery Injuries  ISOPcsk5 (Rattus norvegicus)11556213; 11556213mRNA and protein:increased expression:artery:RGD 
Carotid Artery Injuries  IEP 11556213mRNA and protein:increased expression:artery:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pcsk5  (proprotein convertase subtilisin/kexin type 5)

Genes (Mus musculus)
Pcsk5  (proprotein convertase subtilisin/kexin type 5)

Genes (Homo sapiens)
PCSK5  (proprotein convertase subtilisin/kexin type 5)


Additional Information