RGD Reference Report - A novel dynamin-like protein associates with cytoplasmic vesicles and tubules of the endoplasmic reticulum in mammalian cells. - Rat Genome Database

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A novel dynamin-like protein associates with cytoplasmic vesicles and tubules of the endoplasmic reticulum in mammalian cells.

Authors: Yoon, Y  Pitts, KR  Dahan, S  McNiven, MA 
Citation: Yoon Y, etal., J Cell Biol. 1998 Feb 23;140(4):779-93.
RGD ID: 11535088
Pubmed: PMID:9472031   (View Abstract at PubMed)
PMCID: PMC2141745   (View Article at PubMed Central)

Dynamins are 100-kilodalton guanosine triphosphatases that participate in the formation of nascent vesicles during endocytosis. Here, we have tested if novel dynamin-like proteins are expressed in mammalian cells to support vesicle trafficking processes at cytoplasmic sites distinct from the plasma membrane. Immunological and molecular biological methods were used to isolate a cDNA clone encoding an 80-kilodalton novel dynamin-like protein, DLP1, that shares up to 42% homology with other dynamin-related proteins. DLP1 is expressed in all tissues examined and contains two alternatively spliced regions that are differentially expressed in a tissue-specific manner. DLP1 is enriched in subcellular membrane fractions of cytoplasmic vesicles and endoplasmic reticulum. Morphological studies of DLP1 in cultured cells using either a specific antibody or an expressed green fluorescent protein (GFP)- DLP1 fusion protein revealed that DLP1 associates with punctate cytoplasmic vesicles that do not colocalize with conventional dynamin, clathrin, or endocytic ligands. Remarkably, DLP1-positive structures coalign with microtubules and, most strikingly, with endoplasmic reticulum tubules as verified by double labeling with antibodies to calnexin and Rab1 as well as by immunoelectron microscopy. These observations provide the first evidence that a novel dynamin-like protein is expressed in mammalian cells where it associates with a secretory, rather than endocytic membrane compartment.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
clathrin coat of trans-Golgi network vesicle NOT|part_ofIDA 11535088PMID:9472031ParkinsonsUK-UCL 
cytosol located_inIDA 11535088PMID:9472031ParkinsonsUK-UCL 
endoplasmic reticulum located_inIDA 11535088PMID:9472031ParkinsonsUK-UCL 
Golgi membrane located_inIDA 11535088PMID:9472031ParkinsonsUK-UCL 
perinuclear region of cytoplasm located_inIDA 11535088PMID:9472031ParkinsonsUK-UCL 
plasma membrane NOT|located_inIDA 11535088PMID:9472031ParkinsonsUK-UCL 
secretory vesicle located_inIDA 11535088PMID:9472031ParkinsonsUK-UCL 

Objects Annotated

Genes (Rattus norvegicus)
Dnm1l  (dynamin 1-like)


Additional Information