RGD Reference Report - [Protective effect of adenosine preconditioning against spinal cord ischemia-reperfusion injury in rats]. - Rat Genome Database

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[Protective effect of adenosine preconditioning against spinal cord ischemia-reperfusion injury in rats].

Authors: Fu, Q  Zhou, Z  Li, X  Guo, H  Fan, X  Chen, J  Zhuang, J  Zheng, S  Zhu, P 
Citation: Fu Q, etal., Nan Fang Yi Ke Da Xue Xue Bao. 2014 Jan;34(1):92-5.
RGD ID: 11354961
Pubmed: PMID:24463125   (View Abstract at PubMed)

OBJECTIVE: To study the effect of adenosine preconditioning on cell apoptosis and expressions of glucose-regulated protein (GRP-78) and cysteinyl aspartate-specific protease 12 (caspase-12) in rats with spinal cord ischemia-reperfusion injury. METHODS: Twenty-seven rats were randomized into 3 equal groups and subjected to sham operation (group A), spinal cord ischemia-reperfusion injury (group B), or ischemia-reperfusion injury with adenosine treatment. Spinal cord ischemia-reperfusion injury was induced by cross-clamping of the abdominal aorta inferior to the left renal artery. The spinal cord function was assessed using the Modified Tarlov Scale at 6, 12, and 24 h after reperfusion. At 24 h after reperfusion, histological analysis was carried out with HE staining; cell apoptosis and viability were determined with TUNEL staining, and the expressions of GRP-78 and caspase-12 proteins were determined with Western blotting. RESULTS: HE staining of the spinal cord showed extensive spinal cord injury such as cell edema in group B as compared with group C. Compared with group A, group B showed a significantly increased number of apoptotic cells; the number of apoptotic cells in group B was greater than that in group C. Compared with group B, group C showed significantly increased GRP-78 expression (P<0.01) and decreased caspase-12 expression (P<0.01). CONCLUSION: Adenosine can up-regulate GRP-78 expression and down-regulate caspase-12 expression, and protects the spinal cord against ischemia-reperfusion injury by inhibiting cell apoptosis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Spinal Cord Reperfusion Injury treatmentISOHspa5 (Rattus norvegicus)11354961; 11354961protein:increased expression:spinal cord:RGD 
Spinal Cord Reperfusion Injury treatmentIEP 11354961protein:increased expression:spinal cord:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hspa5  (heat shock protein family A (Hsp70) member 5)

Genes (Mus musculus)
Hspa5  (heat shock protein 5)

Genes (Homo sapiens)
HSPA5  (heat shock protein family A (Hsp70) member 5)


Additional Information