RGD Reference Report - Lipid-mediated transbronchial human interleukin-10 gene transfer decreases acute inflammation associated with allograft rejection in a rat model of lung transplantation.

General

Lipid-mediated transbronchial human interleukin-10 gene transfer decreases acute inflammation associated with allograft rejection in a rat model of lung transplantation.
Authors:	Oishi, H Okada, Y Kikuchi, T Sado, T Oyaizu, T Hoshikawa, Y Suzuki, S Matsumura, Y Kondo, T
Citation:	Oishi H, etal., Transplant Proc. 2007 Jan-Feb;39(1):283-5.
RGD ID:	11049523
Pubmed:	PMID:17275523 (View Abstract at PubMed)
DOI:	DOI:10.1016/j.transproceed.2006.10.207 (Journal Full-text)
BACKGROUND: Transferring genes with immunoregulatory capacity to transplanted organs has the potential to modify allograft rejection (AR). We examined the effect of ex vivo lipid-mediated transbronchial human interleukin-10 (hIL-10) gene transfer on acute AR in a rat model of lung transplantation. METHODS: Left single lung transplantations were performed between a highly histoincompatible rat combination: Brown Norway to Lewis. The extracted donor left lung was intrabronchially instilled with a plasmid encoding hIL-10 or Escherichia coli beta-galactosidase (control), mixed with a cationic lipid. On day 6 posttransplantation, the degree of AR was graded histologically (stages 1-4) based upon pathological categories of inflammation: perivascular, peribronchial, and peribronchiolar lymphocytic infiltrates, edema, intraalveolar hemorrhage, and necrosis. RESULTS: The stage of AR in the IL-10 group (3.1 +/- 0.4) was significantly lower than the control group (3.8 +/- 0.4). Pathological scores for edema, intraalveolar hemorrhage, and necrosis in the IL-10 group (2.3 +/- 0.8, 0.3 +/- 0.5, and 0.3 +/- 0.5, respectively) were also significantly decreased compared with those in the control group (3.2 +/- 0.4, 2.2 +/- 0.8, and 1.2 +/- 0.4, respectively). CONCLUSION: Ex vivo lipid-mediated transbronchial hIL-10 gene transfer attenuated acute inflammation associated with AR in a rat model of lung transplantation.

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
Transplant Rejection	treatment	IMP		11049523		RGD
Transplant Rejection	treatment	ISO	IL10 (Homo sapiens)	11049523; 11049523		RGD

Objects Annotated

Genes (Rattus norvegicus)

Il10 (interleukin 10)

Genes (Mus musculus)

Il10 (interleukin 10)

Genes (Homo sapiens)

IL10 (interleukin 10)

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Lipid-mediated transbronchial human interleukin-10 gene transfer decreases acute inflammation associated with allograft rejection in a rat model of lung transplantation.