RGD Reference Report - Effects of hypothyroidism on expression of CRMP2B and ARPC5 during development of the rat frontal cortex. - Rat Genome Database

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Effects of hypothyroidism on expression of CRMP2B and ARPC5 during development of the rat frontal cortex.

Authors: Liu, CR  Miao, J  Zhang, YL  Liu, YM  Yu, BG 
Citation: Liu CR, etal., Int J Biol Sci. 2013;9(2):209-18. doi: 10.7150/ijbs.5646. Epub 2013 Feb 12.
RGD ID: 11049475
Pubmed: PMID:23459330   (View Abstract at PubMed)
PMCID: PMC3584917   (View Article at PubMed Central)
DOI: DOI:10.7150/ijbs.5646   (Journal Full-text)

Congenital hypothyroidism (CH) can lead to irreversible central nervous system (CNS) damage. However, the pathogenesis of the developmental brain disorders caused by CH has not been completely elucidated. ARPC5 and CRMP2 are closely associated with neurite outgrowth in brain development. Thus, the aim of the present study was to determine whether CRMP2B and ARPC5 expression is altered in the developing cerebral cortex of rats with CH. Control rats and rats with hypothyroidism were sacrificed at birth and at 15 days postpartum. We performed qRT-PCR to detect differences in the crmp2B and arpc5 mRNA expression in the right half of the frontal cortex of these rats. Western blotting was then used to detect differences in CRMP2B and ARPC5 protein expression. Furthermore, immunohistochemical analysis was performed on the left half of the frontal cortex to detect abnormal localization of CRMP2B and ARPC5. Results showed increased expression of the nuclear short isoform of CRMP2B and decreased expression of full-length CRMP2B and ARPC5 in cortical neurons of rats with hypothyroidism. These findings demonstrate that reduced levels of thyroid hormones can inhibit the expression of full-length CRMP2B and ARPC5 and promote nuclear transformation of the short isoform of CRMP2B. CRMP2B and ARPC5 may participate in CNS injury mediated by hypothyroidism by inducing neurite outgrowth inhibition and cytoskeletal protein disorganization.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
congenital hypothyroidism  ISOArpc5 (Rattus norvegicus)11049475; 11049475protein:decreased expression:frontal cortex (rat)RGD 
congenital hypothyroidism  IEP 11049475protein:decreased expression:frontal cortex (rat)RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
orbitofrontal cortex development  IEP 11049475 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Arpc5  (actin related protein 2/3 complex, subunit 5)

Genes (Mus musculus)
Arpc5  (actin related protein 2/3 complex, subunit 5)

Genes (Homo sapiens)
ARPC5  (actin related protein 2/3 complex subunit 5)


Additional Information