RGD Reference Report - Fas antigen (CD95) expression in peripheral blood progenitor cells from patients with leukaemia and lymphoma. - Rat Genome Database

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Fas antigen (CD95) expression in peripheral blood progenitor cells from patients with leukaemia and lymphoma.

Authors: Anthony, RS  Mckelvie, ND  Craig, JI  Parker, AC 
Citation: Anthony RS, etal., Leuk Lymphoma. 1998 Aug;30(5-6):449-58.
RGD ID: 11049157
Pubmed: PMID:9711907   (View Abstract at PubMed)
DOI: DOI:10.3109/10428199809057557   (Journal Full-text)

Fas antigen (CD95) is a cell surface receptor belonging to the tumour necrosis factor/nerve growth factor superfamily and is able to induce apoptosis when triggered by its' natural ligand or an anti-Fas antibody. Fas expression is low on CD34+ bone marrow (BM) progenitor cells, but is increased by various cytokines in vitro. We investigated Fas expression on CD34+ cells from 39 peripheral blood progenitor cell (PBPC) harvests and from 5 normal BM harvests by dual colour flow cytometry to determine if Fas expression was altered during mobilisation. By including calibrated microbeads during flow cytometry, we quantified the number of Fas antigen molecules per cell. A low percentage of PBPC (22%) and normal BM (23%) CD34+ cells expressed Fas antigen. Fas expression varied on CD34+ cells from different diseases and the highest expression was found in ALL (52%). There was a significant three fold increase in the number of Fas molecules/cell expressed on CD34+ cells (PBPC 6,230 molecules/cell, BM 2,236; p = 0.0003). This level of expression was considerably less than that for CD3/CD19 lymphocytes (33,095 molecules/cell) and CD14 monocytes (47,467 molecules/cell) in the PBPC harvest. In conclusion, mobilisation including the use of growth of factors, has minimal effect on CD34 progenitor cell Fas expression.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
acute lymphoblastic leukemia  IEP 11049157protein:increased expression:CD34+ bone marrow cellRGD 
acute lymphoblastic leukemia  ISOFAS (Homo sapiens)11049157; 11049157protein:increased expression:CD34+ bone marrow cellRGD 

Objects Annotated

Genes (Rattus norvegicus)
Fas  (Fas cell surface death receptor)

Genes (Mus musculus)
Fas  (Fas cell surface death receptor)

Genes (Homo sapiens)
FAS  (Fas cell surface death receptor)


Additional Information