RGD Reference Report - Direct interaction between BKCa potassium channel and microtubule-associated protein 1A. - Rat Genome Database

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Direct interaction between BKCa potassium channel and microtubule-associated protein 1A.

Authors: Park, SM  Liu, G  Kubal, A  Fury, M  Cao, L  Marx, SO 
Citation: Park SM, etal., FEBS Lett. 2004 Jul 16;570(1-3):143-8.
RGD ID: 11041045
Pubmed: PMID:15251455   (View Abstract at PubMed)
DOI: DOI:10.1016/j.febslet.2004.06.037   (Journal Full-text)

The BKCa channel, a potassium channel that is allosterically activated by voltage and calcium, is expressed in both excitable and non-excitable cells. The channel plays an important role in regulating membrane excitability. The channel activity can be modulated by post-translational modifications such as phosphorylation. Recently, hippocampal BKCa channels were shown to be directly modulated by assembly/disassembly of the submembranous actin cytoskeleton. Here, we report that the BKCa channel physically interacts with the light chain of microtubule associated protein 1A (MAP1A). The light chain was isolated in a yeast two-hybrid screen of a human brain cDNA library. The specificity of the interaction was demonstrated in biochemical experiments utilizing GST fusion protein pulldown assays and reciprocal co-immunoprecipitations from rat brain. Furthermore, utilizing immunofluorescence, the BKCa channel and MAP1A co-localize in the Purkinje cell layer of the cerebellum. These studies identify a novel interaction between the C-terminal tail of the BKCa channel and the light chain of MAP1A, which enables channel association with and modulation by the cytoskeleton.

Objects referenced in this article
Gene Kcnma1 potassium calcium-activated channel subfamily M alpha 1 Rattus norvegicus
Gene Map1a microtubule-associated protein 1A Rattus norvegicus

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