RGD Reference Report - Pivotal Advance: Eosinophilia in the MES rat strain is caused by a loss-of-function mutation in the gene for cytochrome b(-245), alpha polypeptide (Cyba). - Rat Genome Database

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Pivotal Advance: Eosinophilia in the MES rat strain is caused by a loss-of-function mutation in the gene for cytochrome b(-245), alpha polypeptide (Cyba).

Authors: Mori, M  Li, G  Hashimoto, M  Nishio, A  Tomozawa, H  Suzuki, N  Usami, S  Higuchi, K  Matsumoto, K 
Citation: Mori M, etal., J Leukoc Biol. 2009 Sep;86(3):473-8. doi: 10.1189/jlb.1108715. Epub 2009 Apr 30.
RGD ID: 11040542
Pubmed: PMID:19406829   (View Abstract at PubMed)
DOI: DOI:10.1189/jlb.1108715   (Journal Full-text)

MES is a rat strain that spontaneously develops severe blood eosinophilia as a hereditary trait. Herein, we report that eosinophilia in MES rats is caused by a loss-of-function mutation in the gene for cytochrome b(-245), alpha polypeptide (Cyba; also known as p22(phox)), which is an essential component of the superoxide-generating NADPH oxidase complex. The MES rat has a deletion of four nucleotides, including the 5' splice donor GpT of intron 4 of the Cyba gene. As a consequence of the deletion, a 51-nucleotide sequence of intron 4 is incorporated into the Cyba transcripts. Leukocytes from the MES strain lack both CYBA protein and NADPH oxidase activity. Nevertheless, unlike patients with chronic granulomatous disease, who suffer from infections with pathogens due to similar genetic defects in NADPH oxidase, MES rats retain normal innate immune defense against Staphylococcus aureus infection. This is due to large quantities of peritoneal eosinophils in MES rats, which phagocytose and kill the bacteria. MES rat has a balance defect due to impaired formation of otoconia in the utricles and saccules. Eosinophilia of the MES rat was normalized by introduction of a normal Cyba transgene. The mechanisms by which impairment of NADPH oxidase leads to eosinophilia in the MES rat are elusive. However, our study highlights the essential role of NADPH oxidase in homeostatic regulation of innate immunity beyond conventional microbicidial functions.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Eosinophilia  ISOCyba (Rattus norvegicus)11040542; 11040542DNA:deletion:splice junction:RGD 
Eosinophilia  IAGP 11040542DNA:deletion:splice junction:RGD 
Eosinophilia  IAGP 11040542; 11040542 RGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
absent otoliths  IAGP 11040542; 11040542; 11040542DNA:deletion:splice junction:RGD 
decreased NAD(P)H oxidase activity  IAGP 11040542; 11040542DNA:deletion:splice junction:RGD 
decreased NAD(P)H oxidase activity  IAGP 11040542 RGD 
Objects Annotated

Genes (Rattus norvegicus)
Cyba  (cytochrome b-245 alpha chain)
Cybam1Sdi  (cytochrome b-245 alpha chain;mutant 1,Sdi)

Genes (Mus musculus)
Cyba  (cytochrome b-245, alpha polypeptide)

Genes (Homo sapiens)
CYBA  (cytochrome b-245 alpha chain)

Strains
MES/Slc  (Matsumoto Eosinophilia Shinshu)


Additional Information