RGD Reference Report - Comparative proteomic profiling reveals aberrant cell proliferation in the brain of embryonic Ts1Cje, a mouse model of Down syndrome. - Rat Genome Database

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Comparative proteomic profiling reveals aberrant cell proliferation in the brain of embryonic Ts1Cje, a mouse model of Down syndrome.

Authors: Ishihara, K  Kanai, S  Sago, H  Yamakawa, K  Akiba, S 
Citation: Ishihara K, etal., Neuroscience. 2014 Sep 28;281C:1-15. doi: 10.1016/j.neuroscience.2014.09.039.
RGD ID: 11039463
Pubmed: PMID:25261685   (View Abstract at PubMed)
DOI: DOI:10.1016/j.neuroscience.2014.09.039   (Journal Full-text)

To identify molecular candidates involved in brain disabilities of Ts1Cje, a mouse model of Down syndrome (DS), we performed comparative proteomic analyses. Proteins extracted from the brains of postnatal wild-type (WT) and Ts1Cje mice were analyzed by two-dimensional gel electrophoresis (2-DE). No differences were detected in the proteins expressed in the whole brain between WT and Ts1Cje mice at postnatal day 0 and 3months of age. Five spots with differential expression in the brains of Ts1Cje mice were detected by 2-DE of brain proteins from WT and Ts1Cje embryos at embryonic day 14.5 (E14.5). These differentially expressed proteins in Ts1Cje embryos were identified as calcyclin-binding protein (CACYBP), nucleoside diphosphate kinase-B (NDPK-B), transketolase (TK), pyruvate kinase (PK), and 60S acidic ribosomal protein P0 (RPLP0) by peptide mass fingerprinting. CACYBP and NDPK-B were involved in cell proliferation, whereas TK and PK were associated with energy metabolism. Experiments on cell proliferation, an in vivo bromodeoxyuridine (BrdU)-labeling experiment, and immunohistochemical analysis for phospho-histone H3 (an M-phase marker) demonstrated increased numbers of BrdU-positive and M-phase cells in the ganglionic eminence. Our findings suggest that the dysregulated expression of proteins demonstrated by comparative proteomic analysis could be a factor in increased cell proliferation, which may be associated with abnormalities in DS brain during embryonic life.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Down syndrome  ISORplp0 (Rattus norvegicus)11039463; 11039463protein:increased expression:brainRGD 
Down syndrome  IEP 11039463protein:increased expression:brainRGD 

Objects Annotated

Genes (Rattus norvegicus)
Rplp0  (ribosomal protein lateral stalk subunit P0)

Genes (Mus musculus)
Rplp0  (ribosomal protein lateral stalk subunit P0)

Genes (Homo sapiens)
RPLP0  (ribosomal protein lateral stalk subunit P0)


Additional Information