RGD Reference Report - Mitotic partitioning and selective reorganization of tissue-specific transcription factors in progeny cells. - Rat Genome Database

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Mitotic partitioning and selective reorganization of tissue-specific transcription factors in progeny cells.

Authors: Zaidi, SK  Young, DW  Pockwinse, SM  Javed, A  Lian, JB  Stein, JL  Van Wijnen, AJ  Stein, GS 
Citation: Zaidi SK, etal., Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14852-7. Epub 2003 Dec 1.
RGD ID: 11038801
Pubmed: PMID:14657346   (View Abstract at PubMed)
PMCID: PMC299826   (View Article at PubMed Central)
DOI: DOI:10.1073/pnas.2533076100   (Journal Full-text)

Postmitotic gene expression requires restoration of nuclear organization and assembly of regulatory complexes. The hematopoietic and osteogenic Runx (Cbfa/AML) transcription factors are punctately organized in the interphase nucleus and provide a model for understanding the subnuclear organization of tissue-specific regulatory proteins after mitosis. Here we have used quantitative in situ immunofluorescence microscopy and quantitative image analysis to show that Runx factors undergo progressive changes in cellular localization during mitosis while retaining a punctate distribution. In comparison, the acetyl transferase p300 and acetylated histone H4 remain localized with DNA throughout mitosis while the RNA processing factor SC35 is excluded from mitotic chromatin. Subnuclear organization of Runx foci is completely restored in telophase, and Runx proteins are equally partitioned into progeny nuclei. In contrast, subnuclear organization of SC35 is restored subsequent to telophase. Our results show a sequential reorganization of Runx and its coregulatory proteins that precedes restoration of RNA processing speckles. Thus, mitotic partitioning and spatiotemporal reorganization of regulatory proteins together render progeny cells equivalently competent to support phenotypic gene expression.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
nuclear speck  IDA 11038801 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Srsf2  (serine and arginine rich splicing factor 2)


Additional Information