RGD Reference Report - Regulation of lung oxidative damage by endogenous superoxide dismutase in sepsis. - Rat Genome Database

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Regulation of lung oxidative damage by endogenous superoxide dismutase in sepsis.

Authors: Constantino, L  Goncalves, RC  Giombelli, VR  Tomasi, CD  Vuolo, F  Kist, LW  De Oliveira, GM  Pasquali, MA  Bogo, MR  Mauad, T  Horn A, JR  Melo, KV  Fernandes, C  Moreira, JC  Ritter, C  Dal-Pizzol, F 
Citation: Constantino L, etal., Intensive Care Med Exp. 2014 Dec;2(1):17. doi: 10.1186/2197-425X-2-17. Epub 2014 May 23.
RGD ID: 11035300
Pubmed: PMID:26266917   (View Abstract at PubMed)
PMCID: PMC4513028   (View Article at PubMed Central)
DOI: DOI:10.1186/2197-425X-2-17   (Journal Full-text)

BACKGROUND: The purpose of this research is to study the relationship between superoxide dismutase (SOD) and lung redox state in an animal model of sepsis. METHODS: Sepsis was induced in rats by the cecal ligation and perforation model (CLP). After 3, 6, and 12 h, CLP protein content and expression of SOD1, SOD2, and SOD3 were evaluated, and SOD activity was assessed. Oxidative damage was determined by quantifying nitrotyrosine content. Lung localization of SOD3 was performed by immunohistochemistry. The protective effect of a SOD mimetic on oxidative damage, inflammation, and lung permeability was assessed 12 and 24 h after sepsis induction. RESULTS: Lung levels of SOD1 decreased 3 and 12 h after sepsis, but SOD2 and SOD3 increased, as well as SOD activity. These alterations were not associated with changes in sod gene expression. Nitrotyrosine levels increased 3 and 12 h after sepsis. The administration of a SOD mimetic decreased nitrotyrosine and proinflammatory cytokine levels and improved lung permeability. CONCLUSIONS: SOD2 and SOD3 increased after sepsis induction, but this was insufficient to protect the lung. Treatments based on SOD mimetics could have a role in lung injury associated with sepsis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Sepsis  ISOSod1 (Rattus norvegicus)11035300; 11035300 RGD 
Sepsis  ISOSod2 (Rattus norvegicus)11035300; 11035300mRNA and protein:increased expression:lungRGD 
Sepsis  ISOSod3 (Rattus norvegicus)11035300; 11035300 RGD 
Sepsis  IEP 11035300; 11035300 RGD 
Sepsis  IEP 11035300mRNA and protein:increased expression:lungRGD 

Objects Annotated

Genes (Rattus norvegicus)
Sod1  (superoxide dismutase 1)
Sod2  (superoxide dismutase 2)
Sod3  (superoxide dismutase 3)

Genes (Mus musculus)
Sod1  (superoxide dismutase 1, soluble)
Sod2  (superoxide dismutase 2, mitochondrial)
Sod3  (superoxide dismutase 3, extracellular)

Genes (Homo sapiens)
SOD1  (superoxide dismutase 1)
SOD2  (superoxide dismutase 2)
SOD3  (superoxide dismutase 3)


Additional Information