RGD Reference Report - Protective effect of omega-3 polyunsaturated fatty acids (PUFAs) on sodium nitroprusside-induced nephrotoxicity and oxidative damage in rat kidney. - Rat Genome Database

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Protective effect of omega-3 polyunsaturated fatty acids (PUFAs) on sodium nitroprusside-induced nephrotoxicity and oxidative damage in rat kidney.

Authors: Khan, MW  Priyamvada, S  Khan, SA  Khan, S  Naqshbandi, A  Yusufi, AN 
Citation: Khan MW, etal., Hum Exp Toxicol. 2012 Oct;31(10):1035-49. doi: 10.1177/0960327112444475. Epub 2012 May 1.
RGD ID: 10449175
Pubmed: PMID:22549094   (View Abstract at PubMed)
DOI: DOI:10.1177/0960327112444475   (Journal Full-text)

Sodium nitroprusside (SNP) a nitric oxide (NO) donor has proven toxic effects. Dietary omega-3 polyunsaturated fatty acid (PUFA) has been shown to reduce the severity of numerous ailments. Present study examined whether intake of fish oil (FO)/flaxseed oil (FXO, Omega Nutrition, St Vancouver, Canada) would have protective effect against SNP-induced toxicity. Male Wistar rats (150 +/- 10 g) were used in this study. Initially animals were divided into two groups: one fed on normal diet and the other on 15% FO/FXO for 15 days. On the 16th day, SNP (1.5 mg/kg body weight) was administered intraperitoneally for 7 days daily. After 7 days animals were killed, kidneys were harvested for further analysis. SNP induced nephrotoxicity by increasing serum creatinine and blood urea nitrogen, SNP significantly decreased malate dehydrogenase, glucose-6-phosphatase, fructose-1,6-bisphosphatase and malic enzyme but increased lactate dehydrogenase and glucose-6-phosphate dehydrogenase. Brush border membrane enzymes such as alkaline phosphatase, gamma-glutamyl transpeptidase and leucine amino peptidase were also decreased. The activity of catalase and glutathione peroxidase decreased concomitantly with increased lipid peroxidation, indicating that the significant kidney damage has been inflicted by SNP. Feeding of FO and FXO with SNP ameliorated the changes in various parameters caused by SNP. The results of the present study suggest that omega-3 PUFA-enriched FO and FXO from seafoods and plant sources, respectively, are similarly effective in reducing SNP-induced nephrotoxicity and oxidative damage. Thus, vegetarians who cannot consume FO can have similar health benefits from plant-derived omega-3 PUFA.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
kidney disease treatmentISOG6pd (Rattus norvegicus)10449175; 10449175 RGD 
kidney disease treatmentIEP 10449175 RGD 

Objects Annotated

Genes (Rattus norvegicus)
G6pd  (glucose-6-phosphate dehydrogenase)

Genes (Mus musculus)
G6pdx  (glucose-6-phosphate dehydrogenase X-linked)

Genes (Homo sapiens)
G6PD  (glucose-6-phosphate dehydrogenase)


Additional Information