RGD Reference Report - Developmental exposure of fetal ovaries and fetal germ cells to endometriosis in an endometriosis model causes differential gene expression in the preimplantation embryos of the first-generation and second-generation embryos. - Rat Genome Database

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Developmental exposure of fetal ovaries and fetal germ cells to endometriosis in an endometriosis model causes differential gene expression in the preimplantation embryos of the first-generation and second-generation embryos.

Authors: Birt, JA  Taylor, KH  Davis, JW  Sharpe-Timms, KL 
Citation: Birt JA, etal., Fertil Steril. 2013 Nov;100(5):1436-43. doi: 10.1016/j.fertnstert.2013.07.007. Epub 2013 Aug 15.
RGD ID: 10413877
Pubmed: PMID:23954358   (View Abstract at PubMed)
PMCID: PMC3847911   (View Article at PubMed Central)
DOI: DOI:10.1016/j.fertnstert.2013.07.007   (Journal Full-text)

OBJECTIVE: To characterize multigenerational gene expression anomalies in eight-cell stage embryos associated with developmental exposure to endometriosis. DESIGN: Using an endometriosis model in rats (F0 founder generation) to evaluate gene expression in F1 (fetal exposure) and F2 (fetal germ cell exposure) generation eight-cell stage embryos. SETTING: Laboratory. ANIMAL(S): Endometriosis model in rats (Endo) and controls (Sham). INTERVENTION(S): F0 Endo and Sham rats were bred; half the pregnant rats were killed on gestational day 3 to collect F1 eight-cell stage embryos and the others gestated to term (F1 females). Adult F1 females bred; F2 eight-cell embryos collected. MAIN OUTCOME MEASURE(S): Maintenance of differential gene expression in F1 and F2 generation eight-cell embryos in endometriosis. RESULT(S): Developmental exposure to endometriosis altered the gene signaling pathways, with changes found in apoptosis, the cell cycle process, the response to oxidative stress, negative regulation of molecular function, and RNA processing. The apoptotic genes Diablo, Casp3, Parp1, Cad, and Dnaja3 were increased and the Nfkbia transcripts were decreased in F1 Endo versus F1 Sham embryos. In F2 Endo versus Sham embryos, Casp3 and Cad were statistically significantly increased, and Parp1 and Nfkbia tended to be elevated. CONCLUSION(S): Fetal and germ cell exposure to endometriosis alters apoptotic gene expression in first- and second-generation eight-cell stage embryos, supporting the hypothesis of multigenerational inheritance resulting from exposure to endometriosis in utero.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
endometriosis  ISONfkbia (Rattus norvegicus)10413877; 10413877 RGD 
endometriosis  IEP 10413877 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nfkbia  (NFKB inhibitor alpha)

Genes (Mus musculus)
Nfkbia  (nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha)

Genes (Homo sapiens)
NFKBIA  (NFKB inhibitor alpha)


Additional Information