RGD Reference Report - [Serumimmunological study of moxibustion on helicobacter pylori gastritis in rats]. - Rat Genome Database

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[Serumimmunological study of moxibustion on helicobacter pylori gastritis in rats].

Authors: Peng, Yan  Yi, Shou-Xiang  Feng, Ying-Shuai  Shi, Dong-Mei  Hou, Yan-Ling  Lin, Ya-Ping 
Citation: Peng Y, etal., Zhongguo Zhen Jiu. 2014 Aug;34(8):783-90.
RGD ID: 10413874
Pubmed: PMID:25335260   (View Abstract at PubMed)


OBJECTIVE: To explore the immune mechanism of moxibustion on protecting gastric mucosa injury.
METHODS: Forty healthy SD rats were randomly divided into four groups: a blank group, a model group, a moxibustion acupoint group and a moxibustion non-acupoint group, 10 rats in each one. Eight days before model establishment, moxibustion at "Zusanli" (ST 36), "Zhongwan" (CV 12), "Guanyuan" (CV 4), "Pishu" (BL 20) and "Weishu" (BL 21) was applied in the moxibustion acupoint group while these acupoints' controlled points were selected in the moxibustion non-acupoint group, and no treatment was given in the model group, once a day in three groups for continuous 16 days. The helicobacter pylori (Hp) model was established by intragastric administration of Hp. HE staining microscopic examination was used to observe inflammation severity in gastric mucosa, and enzyme-linked immunosorbent assay (ELISA) was adapted to measure content of heat shock protein (HSP) 72, TNF-alpha and IL-1beta, and real-time quantitative PCR was used to measure the expression of TLR2 mRNA, TLR4 mRNA, CD14 mRNA and MyD88 mRNA in peripheral blood mononuclear cells, and western blot method was used to measure content of NFkappaB and IkappaBalpha in peripheral blood mononuclear cells.
RESULTS: Compared with the blank group, the expression of HP could be seen in the smear of gastric mucosa by Gram's staining in the model group; the inflammation severity score was obviously increased as well as content of serum HSP 72 and TNF-alpha and IL-1beta in gastric tissue; and expression of TLR2, 4 mRNA, CD14 mRNA, MyD88 mRNA, NFkappaB was increased (P < 0.01), but the expression of IkappaBalpha was reduced (P < 0.05). After the moxibustion, the inflammation severity score was reduced in the moxibustion acupoint group, and the content of serum HSP 72 was increased, and the expression of TNF-alpha and IL-1beta in gastric tissue and expression of TLR2 mRNA, TLR4 mRNA, CD14 mRNA, MyD88 mRNA and NFkappaB were reduced (P < 0.01), but the expression of IkappaBalpha was increased (P < 0.05). The differences between the moxibustion non-acupoint group and the model group were not significant (P > 0.05).
CONCLUSION: The pretreatment of moxibustion at acupoints could induce the over expression of serum HSP 72. By combining TLR 2 and 4 receptors to trigger receptor signal transduction pathways, the releases of downstream signal substances are regulated; as a result, the releases of related immune substances are regulated to relieve the gastric mucosa injury of rats with HP gastritis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
gastritis treatmentISONfkbia (Rattus norvegicus)10413874; 10413874associated with Helicobacter InfectionsRGD 
gastritis treatmentIDA 10413874associated with Helicobacter InfectionsRGD 
gastritis treatmentISOTnf (Rattus norvegicus)10413874; 10413874 RGD 
gastritis treatmentIDA 10413874 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nfkbia  (NFKB inhibitor alpha)
Tnf  (tumor necrosis factor)

Genes (Mus musculus)
Nfkbia  (nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha)
Tnf  (tumor necrosis factor)

Genes (Homo sapiens)
NFKBIA  (NFKB inhibitor alpha)
TNF  (tumor necrosis factor)


Additional Information