RGD Reference Report - Reduction of large-conductance Ca(2)(+) -activated K(+) channel with compensatory increase of nitric oxide in insulin resistant rats. - Rat Genome Database

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Reduction of large-conductance Ca(2)(+) -activated K(+) channel with compensatory increase of nitric oxide in insulin resistant rats.

Authors: Li, S  Deng, Z  Wei, L  Liang, L  Ai, W  Shou, X  Chen, X 
Citation: Li S, etal., Diabetes Metab Res Rev. 2011 Jul;27(5):461-9. doi: 10.1002/dmrr.1196.
RGD ID: 10412047
Pubmed: PMID:21425425   (View Abstract at PubMed)
DOI: DOI:10.1002/dmrr.1196   (Journal Full-text)

Cardiovascular disease prevalence and mortality are both increased by insulin resistance, hypertension, and atherosclerosis. The large-conductance Ca(2+)-activated K(+) channel (BK(Ca)) plays a pivotal role in the diastolic function of vascular smooth muscle cells. However, the role of this channel in insulin resistance remains unknown. Male Sprague-Dawley rats were randomly divided into an insulin resistant group and control group. We investigated the BK(Ca) current and subunit expression in myocytes from aortas and mesenteric arteries by Western blot, real-time PCR and the whole-cell patch-clamp methods. BK(Ca) current was decreased in smooth muscle cells in insulin resistant rats, compared with that in control group. Peak BK(Ca) current at + 60 mV was significantly decreased after iberiotoxin (IBTX) perfusion at 100 nmol/L (64.2 +/- 4.7 versus 20.3 +/- 3.5% in thoracic aortas and 65.6 +/- 6.2 versus 29.3 +/- 3.9% in mesenteric arteries, both p < 0.01). However, there was no significant difference in BK(Ca) alpha subunit between the two groups, both at the level of mRNA and protein. BK(Ca) beta 1 subunit expression in aortas and mesenteric arteries from the insulin resistant group was lower than in those from control group. The plasma level of nitric oxide was higher in the insulin resistant group than in the control group. Our results demonstrated that the BK(Ca) channel is decreased both in macrovessels and microvessels in insulin resistant rats. These impairments may be related to the down-regulation of beta1 subunit expression and compensatory increase in plasma nitric oxide levels.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Insulin Resistance  ISOKcnmb1 (Rattus norvegicus)10412047; 10412047mRNA more ...RGD 
Insulin Resistance  IEP 10412047mRNA more ...RGD 

Objects Annotated

Genes (Rattus norvegicus)
Kcnmb1  (potassium calcium-activated channel subfamily M regulatory beta subunit 1)

Genes (Mus musculus)
Kcnmb1  (potassium large conductance calcium-activated channel, subfamily M, beta member 1)

Genes (Homo sapiens)
KCNMB1  (potassium calcium-activated channel subfamily M regulatory beta subunit 1)


Additional Information