RGD Reference Report - Association of potassium channel Kv3.4 subunits with pre- and post-synaptic structures in brainstem and spinal cord. - Rat Genome Database

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Association of potassium channel Kv3.4 subunits with pre- and post-synaptic structures in brainstem and spinal cord.

Authors: Brooke, RE  Atkinson, L  Batten, TF  Deuchars, SA  Deuchars, J 
Citation: Brooke RE, etal., Neuroscience. 2004;126(4):1001-10.
RGD ID: 10411903
Pubmed: PMID:15207333   (View Abstract at PubMed)
DOI: DOI:10.1016/j.neuroscience.2004.03.051   (Journal Full-text)

Voltage-gated K+ channels (Kv) are divided into eight subfamilies (Kv1-8) and play a major role in determining the excitability of neurones. Members of the Kv3 subfamily are highly abundant in the CNS, with each Kv3 gene (Kv3.1-Kv3.4) exhibiting a unique pattern of expression, although single neurones can express more than one subtype. Of the Kv3 subunits relatively little is known of the Kv3.4 subunit distribution in the nervous system, particularly in the brainstem and spinal cord of the rat. We performed immunohistochemistry to determine both the cellular and sub-cellular distribution of the Kv3.4 subunit in these areas. Kv3.4 subunit immunoreactivity (Kv3.4-IR) was widespread, with dense, punctate staining in many regions including the intermediolateral cell column (IML) and the dorsal vagal nucleus (DVN), nucleus ambiguus (NA) and nucleus tractus solitarius (NTS). In the ventral horn a presynaptic location was confirmed by co-localization of Kv3.4-IR with the synaptic vesicle protein, SV2 and also with the glutamate vesicle markers vesicular glutamate transporter (VGluT) 1, VGluT2 or the glycine transporter GlyT2, suggesting a role for the channel in both excitatory and inhibitory neurotransmission. Electron microscopy confirmed a presynaptic terminal location of Kv3.4-IR in the VH, IML, DVN, NA and NTS. Interestingly however, patches of Kv3.4-IR were also revealed postsynaptically in dendritic and somatic structures throughout these areas. This staining was striking due to its localization at synaptic junctions at terminals with morphological features consistent with excitatory functions, suggesting an association with the postsynaptic density. Therefore the pre and postsynaptic localization of Kv3.4-IR suggests a role both in the control of transmitter release and in regulating neuronal excitability.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
dendrite  IDA 10411903 RGD 
glutamatergic synapse is_active_inIDA 10411903PMID:15207333SynGO 
glycinergic synapse is_active_inIDA 10411903PMID:15207333SynGO 
neuronal cell body  IDA 10411903 RGD 
postsynapse  IDA 10411903 RGD 
postsynaptic membrane is_active_inIDA 10411903PMID:15207333SynGO 
presynaptic membrane is_active_inIDA 10411903PMID:15207333SynGO 
synaptic vesicle located_inIDA 10411903 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Kcnc4  (potassium voltage-gated channel subfamily C member 4)


Additional Information