RGD Reference Report - N-acetylcysteine attenuates renal alterations induced by senescence in the rat. - Rat Genome Database

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N-acetylcysteine attenuates renal alterations induced by senescence in the rat.

Authors: Shimizu, MH  Volpini, RA  De Braganca, AC  Campos, R  Canale, D  Sanches, TR  Andrade, L  Seguro, AC 
Citation: Shimizu MH, etal., Exp Gerontol. 2013 Feb;48(2):298-303. doi: 10.1016/j.exger.2012.11.006. Epub 2012 Nov 23.
RGD ID: 10403069
Pubmed: PMID:23183129   (View Abstract at PubMed)
DOI: DOI:10.1016/j.exger.2012.11.006   (Journal Full-text)

The aim of this study was to evaluate the effects of N-acetylcysteine (NAC) on renal function, as well as on sodium and water transporters, in the kidneys of aged rats. Normal, 8-month-old male Wistar rats were treated (n=6) or not (n=6) with NAC (600 mg/L in drinking water) and followed for 16 months. At the end of the follow-up period, we determined inulin clearance, serum thiobarbituric acid reactive substances (TBARS), serum cholesterol, and urinary phosphate excretion. In addition, we performed immunohistochemical staining for p53 and for ED-1-positive cells (macrophages/monocytes), together with Western blotting of kidney tissue for NKCC2, aquaporin 2 (AQP2), urea transporter A1 (UT-A1) and Klotho protein. At baseline, the two groups were similar in terms of creatinine clearance, proteinuria, cholesterol, and TBARS. At the end of the follow-up period, NAC-treated rats presented greater inulin clearance and reduced proteinuria, as well as lower serum cholesterol, serum TBARS, and urinary phosphate excretion, in comparison with untreated rats. In addition, NAC-treated rats showed upregulated expression of NKCC2, AQP2, and UT-A1; elevated Klotho protein expression, low p53 expression, and few ED-1 positive cells. In conclusion, we attribute these beneficial effects of NAC (the significant improvements in inulin clearance and in the expression of NKCC2, AQP2, and UT-A1) to its ability to decrease oxidative stress, inhibit p53 expression, minimize kidney inflammation, and stimulate Klotho expression.

Objects referenced in this article
Gene Kl Klotho Rattus norvegicus

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