RGD Reference Report - Klotho reduces apoptosis in experimental ischaemic acute renal failure. - Rat Genome Database

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Klotho reduces apoptosis in experimental ischaemic acute renal failure.

Authors: Sugiura, H  Yoshida, T  Tsuchiya, K  Mitobe, M  Nishimura, S  Shirota, S  Akiba, T  Nihei, H 
Citation: Sugiura H, etal., Nephrol Dial Transplant. 2005 Dec;20(12):2636-45. Epub 2005 Oct 4.
RGD ID: 10403064
Pubmed: PMID:16204278   (View Abstract at PubMed)
DOI: DOI:10.1093/ndt/gfi165   (Journal Full-text)

BACKGROUND: Klotho is associated with the suppression of several ageing phenotypes. Because high klotho gene expression was detected in the kidney and several studies have found altered expression in animal models, we explored the physiological relevance of klotho expression in the kidney under renal ischemia reperfusion injury (IRI). METHODS: Male Wistar rats were subjected to bilateral renal ischemia or sham operation, followed by reperfusion for 6, 12 or 24 h, or 2 to 10 days. Renal expression of klotho was assessed by real-time PCR or Western blotting. Creatinine levels were determined. Immunohistochemical studies and TUNEL staining were performed. An adenovirus harbouring the mouse klotho gene (ad-kl) was intravenously administered to one group of rats before renal IRI. RESULTS: Renal klotho mRNA and protein expressions were significantly reduced in IRI rats the first day after ischemia. Pre-treatment with ad-kl resulted in a robust induction of klotho mRNA and protein in the liver but not in the kidney. Ad-kl gene transfer improved serum creatinine and the histological changes. Apoptosis induced by IRI was attenuated following ad-kl administration. CONCLUSION: The data suggest klotho to be involved in the pathophysiology of IRI. Downregulation of renal klotho exacerbates ischaemic acute renal failure, and klotho gene induction has therapeutic potential in managing ischaemic renal damage.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Kidney Reperfusion Injury treatmentISOKl (Mus musculus)10403064; 10403064 RGD 
Kidney Reperfusion Injury  ISOKl (Rattus norvegicus)10403064; 10403064mRNA and protein:decreased expression:renal tubule:RGD 
Kidney Reperfusion Injury  IEP 10403064mRNA and protein:decreased expression:renal tubule:RGD 
Kidney Reperfusion Injury treatmentIMP 10403064 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Kl  (Klotho)

Genes (Mus musculus)
Kl  (klotho)

Genes (Homo sapiens)
KL  (klotho)


Additional Information