RGD Reference Report - Interaction of huntingtin-associated protein with dynactin P150Glued. - Rat Genome Database

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Interaction of huntingtin-associated protein with dynactin P150Glued.

Authors: Li, SH  Gutekunst, CA  Hersch, SM  Li, XJ 
Citation: Li SH, etal., J Neurosci. 1998 Feb 15;18(4):1261-9.
RGD ID: 10403028
Pubmed: PMID:9454836   (View Abstract at PubMed)
PMCID: PMC6792727   (View Article at PubMed Central)

Huntingtin is the protein product of the gene for Huntington's disease (HD) and carries a polyglutamine repeat that is expanded in HD (>36 units). Huntingtin-associated protein (HAP1) is a neuronal protein and binds to huntingtin in association with the polyglutamine repeat. Like huntingtin, HAP1 has been found to be a cytoplasmic protein associated with membranous organelles, suggesting the existence of a protein complex including HAP1, huntingtin, and other proteins. Using the yeast two-hybrid system, we found that HAP1 also binds to dynactin P150(Glued) (P150), an accessory protein for cytoplasmic dynein that participates in microtubule-dependent retrograde transport of membranous organelles. An in vitro binding assay showed that both huntingtin and P150 selectively bound to a glutathione transferase (GST)-HAP1 fusion protein. An immunoprecipitation assay demonstrated that P150 and huntingtin coprecipitated with HAP1 from rat brain cytosol. Western blot analysis revealed that HAP1 was enriched in rat brain microtubules and comigrated with P150 and huntingtin in sucrose gradients. Immunofluorescence showed that transfected HAP1 colocalized with P150 and huntingtin in human embryonic kidney (HEK) 293 cells. We propose that HAP1, P150, and huntingtin are present in a protein complex that may participate in dynein-dynactin-associated intracellular transport.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding  IPIHtt (Rattus norvegicus)10403028 RGD 
protein binding  IPIHap1 (Rattus norvegicus)10403028 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hap1  (huntingtin-associated protein 1)
Htt  (huntingtin)


Additional Information