RGD Reference Report - The effects of recombinant human insulin-like growth factor (rhIGF)-1 and rhIGF-1/IGF binding protein-3 administration on rat osteopenia induced by ovariectomy with concomitant bilateral sciatic neurectomy. - Rat Genome Database

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The effects of recombinant human insulin-like growth factor (rhIGF)-1 and rhIGF-1/IGF binding protein-3 administration on rat osteopenia induced by ovariectomy with concomitant bilateral sciatic neurectomy.

Authors: Narusawa, K  Nakamura, T  Suzuki, K  Matsuoka, Y  Lee, LJ  Tanaka, H  Seino, Y 
Citation: Narusawa K, etal., J Bone Miner Res. 1995 Dec;10(12):1853-64.
RGD ID: 10402812
Pubmed: PMID:8619365   (View Abstract at PubMed)
DOI: DOI:10.1002/jbmr.5650101204   (Journal Full-text)

We compared the effect of administration of recombinant human insulin-like growth factor-1 (rhIGF-1) alone or the rhIGF-1/IGF binding protein-3 (IGFBP-3) complex on osteopenia in rats. Female Sprague-Dawley rats (8 months old) underwent combined ovariectomy and bilateral sciatic neurectomy (OVX-NX) or sham operation only. After 2 months, the OVX-NX animals were injected subcutaneously with rhIGF-1 alone or with rhIGF-1A IGFBP-3 equimolar complex for 4 weeks. The IGF-1 contents and dose were 0.3 mg/kg of body weight (BW) three times/week, 3 mg/kg of BW once/week, or 3 mg/kg of BW three times /week. At the end of the experiment, the 4th and 5th lumbar vertebrae (L4, L5) and the proximal tibiae were removed after tetracycline labeling, and histomorphometrical analyses were performed on undecalcified sections using Villanueva's staining. The cancellous bone volume at L5 significantly increased by thickening of the trabecular width in rats treated with the complex. However, the increase in the values at the proximal tibia was not significant. The bone formation rates (BFR/BS) in the lumbar vertebrae of rats treated with the complex three times a week at doses of 0.3 mg/kg of BW and 3 mg/kg of BW were both significantly increased but the parameter increase was less marked with the dose of 3 mg/kg of BW once/week. The BFR/BS did not increase significantly in animals treated with IGF-1 alone. These findings clearly demonstrated that the effect of systemically administered rhIGF-1 on bone formation was markedly potentiated when combined with IGFBP-3 in estrogen deficiency combined with reduced activity. The action of IGF-1 was less potent on the bone in paralyzed limbs. The action of rhIGF-1/IGFBP-3 on trabecular bone appeared to depend not only on the dose but also on the frequency of administration and the parts of the skeleton in rats.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Metabolic Bone Diseases treatmentIDA 10402812; 10402812 RGD 
Metabolic Bone Diseases treatmentISOIGF1 (Homo sapiens)10402812; 10402812 RGD 
Metabolic Bone Diseases treatmentISOIGFBP3 (Homo sapiens)10402812; 10402812 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Igf1  (insulin-like growth factor 1)
Igfbp3  (insulin-like growth factor binding protein 3)

Genes (Mus musculus)
Igf1  (insulin-like growth factor 1)
Igfbp3  (insulin-like growth factor binding protein 3)

Genes (Homo sapiens)
IGF1  (insulin like growth factor 1)
IGFBP3  (insulin like growth factor binding protein 3)


Additional Information