RGD Reference Report - HSP90 inhibition by 17-DMAG attenuates oxidative stress in experimental atherosclerosis. - Rat Genome Database

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HSP90 inhibition by 17-DMAG attenuates oxidative stress in experimental atherosclerosis.

Authors: Madrigal-Matute, J  Fernandez-Garcia, CE  Gomez-Guerrero, C  Lopez-Franco, O  Munoz-Garcia, B  Egido, J  Blanco-Colio, LM  Martin-Ventura, JL 
Citation: Madrigal-Matute J, etal., Cardiovasc Res. 2012 Jul 1;95(1):116-23. doi: 10.1093/cvr/cvs158. Epub 2012 Apr 30.
RGD ID: 10402548
Pubmed: PMID:22547655   (View Abstract at PubMed)
DOI: DOI:10.1093/cvr/cvs158   (Journal Full-text)

AIMS: Reactive oxygen species (ROS) participate in atherogenesis through different mechanisms including oxidative stress and inflammation. Proteins implicated in both processes, such as mitogen-activated protein kinase kinase (MEK) and some NADPH oxidase (NOX) subunits, are heat shock protein-90 (HSP90) client proteins. In this work, we investigated the antioxidant properties of the HSP90 inhibitor, 17-dimethylaminoethylamino-17-demethoxygeldanamycin (17-DMAG) in experimental atherosclerosis. METHODS AND RESULTS: Treatment of ApoE(-/-) mice with 17-DMAG (2 mg/kg every 2 days for 10 weeks) decreased ROS levels and extracellular signal-regulated kinase (ERK) activation in aortic plaques compared with control animals. Accordingly, treatment of rat vascular smooth muscle cells (VSMCs) with 17-DMAG increased HSP27 and HSP70 and inhibited ERK activation. Interestingly, 17-DMAG diminished NADPH oxidase dependent ROS production in VSMCs and monocytes. In addition, a marked reduction in NADPH oxidase dependent ROS production was observed with HSP90siRNA and the opposite pattern with HSP70siRNA. 17-DMAG also diminished the expression of Nox1 and Nox organizer-1 (Noxo1) in VSMCs and monocytes. Interestingly, 17-DMAG was able to modulate ROS-induced monocyte to macrophage differentiation. Finally, higher expression of Nox1 and Noxo1 was found in the inflammatory region of human atherosclerotic plaques, colocalizing with VSMCs, macrophages, and ROS-producing cells. CONCLUSION: Our results suggest that HSP90 inhibitors interfere with oxidative stress and modulate experimental atherosclerosis development through reduction in pro-oxidative factors.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of reactive oxygen species biosynthetic process  IMP 10402548 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hspd1  (heat shock protein family D (Hsp60) member 1)


Additional Information