RGD Reference Report - Identification of mitochondria translation elongation factor Tu as a contributor to oxidative damage of postburn myocardium. - Rat Genome Database

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Identification of mitochondria translation elongation factor Tu as a contributor to oxidative damage of postburn myocardium.

Authors: Zhang, DX  Yan, H  Hu, JY  Zhang, JP  Teng, M  Tong, DL  Xiang, F  Zhang, Q  Fang, YD  Liang, GP  Huang, YS 
Citation: Zhang DX, etal., J Proteomics. 2012 Dec 21;77:469-79. doi: 10.1016/j.jprot.2012.09.024. Epub 2012 Oct 2.
RGD ID: 10401126
Pubmed: PMID:23041468   (View Abstract at PubMed)
DOI: DOI:10.1016/j.jprot.2012.09.024   (Journal Full-text)

Mitochondrial damage plays an important role in mediating postburn cardiac injury. To elucidate the pivotal mitochondrial proteins and pathways underlying postburn cardiac injury, mitochondria were purified from control and postburn rat hearts. 2-dimensional gel electrophoresis (2-DE) and HPLC-chip-MS/MS analyses revealed 9 differentially expressed proteins, 3 of which were further validated by Western blotting. The differential expression of these mitochondrial proteins was accompanied by increased levels of oxidative cardiac damage and decreased levels of cardiac output. One of the differentially expressed proteins, mitochondria translation elongation factor Tu (EF-Tumt), was hypothesized to contribute crucially to postburn oxidative cardiac damage. The small interfering RNA (siRNA)-mediated downregulation of EF-Tumt in cultured rat cardiomyocytes increased reactive oxygen species (ROS) generation and protein carbonyl levels, and led to cell damage. The potential pathway of this process was associated with respiratory chain complex I deficiency. Together, these results demonstrate the mitochondrial responses to severe burn, and indicate a pathway by which decreased EF-Tumt expression mediates oxidative damage in postburn myocardium.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Burns  ISOEef1a1 (Rattus norvegicus)10401126; 10401126protein:decreased expression:heart and mitochondrion:RGD 
Burns  IEP 10401126protein:decreased expression:heart and mitochondrion:RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of reactive oxygen species biosynthetic process  IMP 10401126 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Eef1a1  (eukaryotic translation elongation factor 1 alpha 1)

Genes (Mus musculus)
Eef1a1  (eukaryotic translation elongation factor 1 alpha 1)

Genes (Homo sapiens)
EEF1A1  (eukaryotic translation elongation factor 1 alpha 1)


Additional Information