RGD Reference Report - Sulfonylurea receptors regulate the channel pore in ATP-sensitive potassium channels via an intersubunit salt bridge. - Rat Genome Database

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Sulfonylurea receptors regulate the channel pore in ATP-sensitive potassium channels via an intersubunit salt bridge.

Authors: Lodwick, D  Rainbow, RD  Rubaiy, HN  Al Johi, M  Vuister, GW  Norman, RI 
Citation: Lodwick D, etal., Biochem J. 2014 Dec 15;464(3):343-54. doi: 10.1042/BJ20140273.
RGD ID: 10400872
Pubmed: PMID:25236767   (View Abstract at PubMed)
DOI: DOI:10.1042/BJ20140273   (Journal Full-text)

ATP-sensitive potassium channels play key roles in many tissues by coupling metabolic status to membrane potential. In contrast with other potassium channels, the pore-forming Kir6 subunits must co-assemble in hetero-octameric complexes with ATP-binding cassette (ABC) family sulfonylurea receptor (SUR) subunits to facilitate cell surface expression. Binding of nucleotides and drugs to SUR regulates channel gating but how these responses are communicated within the complex has remained elusive to date. We have now identified an electrostatic interaction, forming part of a functional interface between the cytoplasmic nucleotide-binding domain-2 of SUR2 subunits and the distal C-terminus of Kir6 polypeptides that determines channel response to nucleotide, potassium channel opener and antagonist. Mutation of participating residues disrupted physical interaction and regulation of expressed channels, properties that were restored in paired charge-swap mutants. Equivalent interactions were identified in Kir6.1- and Kir6.2-containing channels suggesting a conserved mechanism of allosteric regulation.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
inward rectifying potassium channel located_inIPI 10400872; 10400872; 10400872; 10400872PMID:25236767ComplexPortal 

Objects Annotated

Genes (Rattus norvegicus)
Abcc9  (ATP binding cassette subfamily C member 9)
Abcc9_v1  (ATP binding cassette subfamily C member 9, variant 1)
Abcc9_v2  (ATP binding cassette subfamily C member 9, variant 2)
Kcnj11  (potassium inwardly-rectifying channel, subfamily J, member 11)


Additional Information