RGD Reference Report - Direct interaction of the calcium sensor protein synaptotagmin I with a cytoplasmic domain of the alpha1A subunit of the P/Q-type calcium channel. - Rat Genome Database

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Direct interaction of the calcium sensor protein synaptotagmin I with a cytoplasmic domain of the alpha1A subunit of the P/Q-type calcium channel.

Authors: Charvin, N  L'eveque, C  Walker, D  Berton, F  Raymond, C  Kataoka, M  Shoji-Kasai, Y  Takahashi, M  De Waard, M  Seagar, MJ 
Citation: Charvin N, etal., EMBO J. 1997 Aug 1;16(15):4591-6.
RGD ID: 10054440
Pubmed: PMID:9303303   (View Abstract at PubMed)
PMCID: PMC1170085   (View Article at PubMed Central)
DOI: DOI:10.1093/emboj/16.15.4591   (Journal Full-text)

Synaptotagmins are synaptic vesicle proteins containing two calcium-binding C2 domains which are involved in coupling calcium influx through voltage-gated channels to vesicle fusion and exocytosis of neurotransmitters. The interaction of synaptotagmins with native P/Q-type calcium channels was studied in solubilized synaptosomes from rat cerebellum. Antibodies against synaptotagmins I and II, but not IV co-immunoprecipitated [125I]omega-conotoxin MVIIC-labelled calcium channels. Direct interactions were studied between in vitro-translated [35S]synaptotagmin I and fusion proteins containing cytoplasmic loops of the alpha1A subunit (BI isoform). Gel overlay revealed the association of synaptotagmin I with a single region (residues 780-969) located in the intracellular loop connecting homologous domains II and III. Saturable calcium-independent binding occurred with equilibrium dissociation constants of 70 nM and 340 nM at 4 degrees C and pH 7.4, and association was blocked by addition of excess recombinant synaptotagmin I. Direct synaptotagmin binding to the pore-forming subunit of the P/Q-type channel may optimally locate the calcium-binding sites that initiate exocytosis within a zone of voltage-gated calcium entry.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Cacna1a  (calcium voltage-gated channel subunit alpha1 A)
Syt1  (synaptotagmin 1)
Syt2  (synaptotagmin 2)


Additional Information