RGD Reference Report - Immunohistological localization of the myelinating cell-specific receptor LP(A1). - Rat Genome Database

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Immunohistological localization of the myelinating cell-specific receptor LP(A1).

Authors: Cervera, P  Tirard, M  Barron, S  Allard, J  Trottier, S  Lacombe, J  Daumas-Duport, C  Sokoloff, P 
Citation: Cervera P, etal., Glia. 2002 Apr 15;38(2):126-36.
RGD ID: 10054294
Pubmed: PMID:11948806   (View Abstract at PubMed)

LP(A1) (also termed Edg-2 or VZG-1) is a G-protein-coupled receptor for lysophosphatidic acid and its gene transcripts have been found selectively expressed by mature myelin-producing cells. We have raised in rabbit a polyclonal antibody against a sequence unique to LP(A1) and common to rat, mouse, and human orthologues. In Western blots, LP(A1) immunoreactivity appeared as 44-53 kDa bands in extracts from recombinant RH7777 cells expressing LP(A1), mouse purified oligodendrocytes, or human white matter, but not from wild-type RH7777 cells or purified astrocytes. In glial cultures, LP(A1) immunoreactivity was restricted to oligodendrocytes, appeared at cell membrane and processes, colocalized with myelin basic protein, and appeared before myelin/oligodendrocyte glycoprotein. In slices of rat and human brains, LP(A1) immunoreactivity was found in myelinated tracts, as well as in oligodendrocyte somata and their myelinating fibers. Immunoreactivities of LP(A1) and myelin basic protein colocalized in the brain, but oligodendrocyte soma showed stronger signals for LP(A1) than myelinated fibers, whereas the reverse was true for myelin basic protein. These results strengthen the view that LP(A1) is involved in myelin formation or maintenance.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
oligodendrocyte development  IEP 10054294 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
neuronal cell body  IDA 10054294 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Lpar1  (lysophosphatidic acid receptor 1)

Objects referenced in this article
Gene Bud31 BUD31 homolog Rattus norvegicus

Additional Information