RGD Reference Report - Shootin1 interacts with actin retrograde flow and L1-CAM to promote axon outgrowth. - Rat Genome Database

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Shootin1 interacts with actin retrograde flow and L1-CAM to promote axon outgrowth.

Authors: Shimada, T  Toriyama, M  Uemura, K  Kamiguchi, H  Sugiura, T  Watanabe, N  Inagaki, N 
Citation: Shimada T, etal., J Cell Biol. 2008 Jun 2;181(5):817-29. doi: 10.1083/jcb.200712138.
RGD ID: 10054086
Pubmed: PMID:18519736   (View Abstract at PubMed)
PMCID: PMC2396814   (View Article at PubMed Central)
DOI: DOI:10.1083/jcb.200712138   (Journal Full-text)

Actin polymerizes near the leading edge of nerve growth cones, and actin filaments show retrograde movement in filopodia and lamellipodia. Linkage between actin filament retrograde flow and cell adhesion molecules (CAMs) in growth cones is thought to be one of the mechanisms for axon outgrowth and guidance. However, the molecular basis for this linkage remains elusive. Here, we show that shootin1 interacts with both actin filament retrograde flow and L1-CAM in axonal growth cones of cultured rat hippocampal neurons, thereby mediating the linkage between them. Impairing this linkage, either by shootin1 RNA interference or disturbing the interaction between shootin1 and actin filament flow, inhibited L1-dependent axon outgrowth, whereas enhancing the linkage by shootin1 overexpression promoted neurite outgrowth. These results strengthen the actin flow-CAM linkage model ("clutch" model) for axon outgrowth and suggest that shootin1 is a key molecule involved in this mechanism.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of axon extension involved_inIMP 10054086PMID:18519736UniProt 
positive regulation of axon extension involved_inIDA 10054086PMID:18519736UniProt 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
axonal growth cone located_inIDA 10054086; 10054086PMID:18519736UniProt 
filopodium located_inIDA 10054086PMID:18519736UniProt 
lamellipodium located_inIDA 10054086PMID:18519736UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cell adhesion molecule binding enablesIPIUniProtKB:Q0569510054086PMID:18519736UniProt 
protein binding enablesIPIUniProtKB:A0MZ6710054086PMID:18519736UniProt 

Objects Annotated

Genes (Rattus norvegicus)
L1cam  (L1 cell adhesion molecule)
Shtn1  (shootin 1)


Additional Information