RGD Reference Report - Propylthiouracil-induced hypothyroidism delays apoptosis during the first wave of spermatogenesis. - Rat Genome Database

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Propylthiouracil-induced hypothyroidism delays apoptosis during the first wave of spermatogenesis.

Authors: Silva, D  Lizama, C  Tapia, V  Moreno, RD 
Citation: Silva D, etal., Biol Res. 2011;44(2):181-8. doi: /S0716-97602011000200010. Epub 2011 Sep 20.
RGD ID: 10053713
Pubmed: PMID:22513421   (View Abstract at PubMed)
DOI: DOI:/S0716-97602011000200010   (Journal Full-text)

Mammalian germ cell apoptosis plays a key role in controlling the correct number of germ cells supported by Sertoli cells during the first wave of spermatogenesis in mammalian puberty. However, little is known about hormonal factors that could influence the rate of germ cell apoptosis during puberty or adulthood. In this work we evaluate germ cell apoptosis under hypothyroidism induced by goitrogen propylthiouracil (PTU) during the first wave of spermatogenesis. Neonatally administered PTU promoted a delay in the differentiation of Sertoli cells as evaluated by the expression of clusterin using immunohistochemistry and RT-PCR. Clusterin had different expression levels in control and PTU-treated animals, but under both conditions the highest levels were found in 35-day-old rats. In addition, clusterin displayed a cytoplasmic localization in control testes, but appeared located in the nucleus in PTU-treated animals. The wave of apoptosis (determined by caspase activity and quantification of apoptotic cells) characteristic of the first round of spermatogenesis was delayed by at least 10 days in these animals. The expression levels of proapoptotic genes like BAX or BAD were different between control and PTU-treated rats; although in both groups the highest level was found at the same age (days). Thus our results indicate that the characteristic pubertal apoptotic wave during rat spermatogenesis is delayed in neonatal hypothyroid rats.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
hypothyroidism  ISOBad (Rattus norvegicus)10053713; 10053713 RGD 
hypothyroidism  IEP 10053713 RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
spermatogenesis  IEP 10053713 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Bad  (BCL2-associated agonist of cell death)

Genes (Mus musculus)
Bad  (BCL2-associated agonist of cell death)

Genes (Homo sapiens)
BAD  (BCL2 associated agonist of cell death)


Additional Information