RGD Reference Report - Effect of miR-23 on oxidant-induced injury in human retinal pigment epithelial cells. - Rat Genome Database

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Effect of miR-23 on oxidant-induced injury in human retinal pigment epithelial cells.

Authors: Lin, H  Qian, J  Castillo, AC  Long, B  Keyes, KT  Chen, G  Ye, Y 
Citation: Lin H, etal., Invest Ophthalmol Vis Sci. 2011 Aug 9;52(9):6308-14. doi: 10.1167/iovs.10-6632.
RGD ID: 10053591
Pubmed: PMID:21693609   (View Abstract at PubMed)
DOI: DOI:10.1167/iovs.10-6632   (Journal Full-text)

PURPOSE: Micro(mi)RNAs negatively regulate a wide variety of genes through degradation or posttranslational inhibition of their target genes. The purpose of this study was to investigate the role of miR-23a in modulating RPE cell survival and gene expression in response to oxidative damage. METHODS: The expression level of miR-23a was measured in macular retinal pigment epithelial (RPE) cells of donor eyes with aged-related macular degeneration (AMD) and age-matched normal eyes by using qRT-PCR. Cultured human ARPE-19 cells were transfected with miR-23a mimic or inhibitor. Cell viability was assessed by the MTT assay. Apoptosis was determined by incubating cells with hydrogen peroxide (H(2)O(2)) or t-butylhydroperoxide (tBH). Caspase-3 activity and DNA fragmentation were measured by enzyme-linked immunosorbent assays. The protein relevant to apoptosis, such as Fas expression level, was analyzed by Western blot analysis. RESULTS: miR-23a expression was significantly downregulated in macular RPE cells from AMD eyes. H(2)O(2)-induced ARPE-19 cell death and apoptosis were increased by an miR-23a inhibitor and decreased by an miR-23a mimic. Computational analysis found a putative target site of miR-23a in the 3'UTR of Fas mRNA, which was verified by a luciferase reporter assay. Forced overexpression of miR-23a decreased H(2)O(2) or tBH-induced Fas upregulation, and this effect was blocked by downregulation of miR-23a. CONCLUSIONS: The protection of RPE cells against oxidative damage is afforded by miR-23a through regulation of Fas, which may be a novel therapeutic target in retinal degenerative diseases.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
macular degeneration  IEP 10053591RNA:decreased expression:retinal pigment epithelial cell:RGD 
macular degeneration  ISOMIR23A (Homo sapiens)10053591; 10053591RNA:decreased expression:retinal pigment epithelial cell:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mir23a  (microRNA 23a)

Genes (Mus musculus)
Mir23a  (microRNA 23a)

Genes (Homo sapiens)
MIR23A  (microRNA 23a)


Additional Information