RGD Reference Report - MicroRNA-145 protects cardiomyocytes against hydrogen peroxide (H(2)O(2))-induced apoptosis through targeting the mitochondria apoptotic pathway. - Rat Genome Database

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MicroRNA-145 protects cardiomyocytes against hydrogen peroxide (H(2)O(2))-induced apoptosis through targeting the mitochondria apoptotic pathway.

Authors: Li, R  Yan, G  Li, Q  Sun, H  Hu, Y  Sun, J  Xu, B 
Citation: Li R, etal., PLoS One. 2012;7(9):e44907. doi: 10.1371/journal.pone.0044907. Epub 2012 Sep 18.
RGD ID: 10047368
Pubmed: PMID:23028672   (View Abstract at PubMed)
PMCID: PMC3445575   (View Article at PubMed Central)
DOI: DOI:10.1371/journal.pone.0044907   (Journal Full-text)

MicroRNAs, a class of small and non-encoding RNAs that transcriptionally or post-transcriptionally modulate the expression of their target genes, has been implicated as critical regulatory molecules in many cardiovascular diseases, including ischemia/reperfusion induced cardiac injury. Here, we report microRNA-145, a tumor suppressor miRNA, can protect cardiomyocytes from hydrogen peroxide H(2)O(2)-induced apoptosis through targeting the mitochondrial pathway. Quantitative real-time PCR (qPCR) demonstrated that the expression of miR-145 in either ischemia/reperfused mice myocardial tissues or H(2)O(2)-treated neonatal rat ventricle myocytes (NRVMs) was markedly down-regulated. Over-expression of miR-145 significantly inhibited the H(2)O(2)-induced cellular apoptosis, ROS production, mitochondrial structure disruption as well as the activation of key signaling proteins in mitochondrial apoptotic pathway. These protective effects of miR-145 were abrogated by over-expression of Bnip3, an initiation factor of the mitochondrial apoptotic pathway in cardiomyocytes. Finally, we utilized both luciferase reporter assay and western blot analysis to identify Bnip3 as a direct target of miR-145. Our results suggest miR-145 plays an important role in regulating mitochondrial apoptotic pathway in heart challenged with oxidative stress. MiR-145 may represent a potential therapeutic target for treatment of oxidative stress-associated cardiovascular diseases, such as myocardial ischemia/reperfusion injury.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Myocardial Ischemia  ISOMir145a (Mus musculus)10047368; 10047368miRNA:decreased expression:myocardium (mouse)RGD 
Myocardial Ischemia  IEP 10047368miRNA:decreased expression:myocardium (mouse)RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to hydroperoxide  IEP 10047368 RGD 
positive regulation of cardiac muscle cell apoptotic process involved_inIDA 10047368PMID:23028672BHF-UCL 

Objects Annotated

Genes (Rattus norvegicus)
Bnip3  (BCL2 interacting protein 3)
Mir145  (microRNA 145)

Genes (Mus musculus)
Mir145a  (microRNA 145a)

Genes (Homo sapiens)
MIR145  (microRNA 145)


Additional Information