RGD Reference Report - CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials. - Rat Genome Database

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CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials.

Authors: Ossipow, V  Descombes, P  Schibler, U 
Citation: Ossipow V, etal., Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8219-23.
RGD ID: 10047356
Pubmed: PMID:8367486   (View Abstract at PubMed)
PMCID: PMC47320   (View Article at PubMed Central)

The CCAAT/enhancer-binding protein (C/EBP) alpha is a leucine zipper protein that is preferentially expressed in certain cell types, such as adipocytes and hepatocytes. Here we show that C/EBP alpha mRNA is translated into two major proteins, C/EBP-42 and C/EBP-30, that differ in their content of N-terminal amino acid sequences. These results are best explained by a ribosome-scanning mechanism in which a fraction of ribosomes ignore the first two AUGs and initiate translation at an AUG located 351 nt downstream of the first one. Because C/EBP-30, the translation product initiated at the third AUG, is devoid of the potent transcription-activation domain contained in C/EBP-42, the former protein stimulates transcription from the mouse albumin promoter much less efficiently than the latter. The gene encoding the liver-enriched transcriptional-activator protein LAP (C/EBP-beta) has also been shown to issue two proteins, LAP and the liver-enriched transcriptional-inhibitory protein LIP, with different transcription-activation potentials. The production of multiple proteins from a single mRNA is not only shared between different C/EBP family members but also appears to be conserved in vertebrate evolution.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Cebpa  (CCAAT/enhancer binding protein alpha)
Cebpb  (CCAAT/enhancer binding protein beta)


Additional Information